HIV Vaccines Comprising One or More Population Episensus Antigens

ABSTRACT

Provided herein are HIV-1 vaccines comprising a carrier and a population episensus antigen determined using the EpiGraph approach. Also provided are HIV-1 vaccines comprising a carrier, a population episensus antigen, and a tailored antigen. Also provided are methods of designing and producing an HIV-1 vaccine for a subject comprising designing vaccine antigens to optimally cover the diversity within a geographic area using an antigen amino acid sequence generated using the EpiGraph approach, and producing said designed vaccine antigen. Also provided are methods of inducing an effector memory T cell response comprising designing the one or more EpiGraph amino acid sequences, producing a vaccine comprising the one or more EpiGraph amino acid sequences and a vector, and administering the vaccine to a subject. Further provided are methods of treating HIV-1 in a subject comprising administering an effective amount of the described HIV-1 vaccines to the subject in need thereof.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority benefit to U.S. Provisional Patent Application Ser. No. 62/059,497, filed Oct. 3, 2014, and U.S. Provisional Patent Application Ser. No. 62/059,506, filed Oct. 3, 2014, each of which is hereby incorporated by reference herein in its entirety.

REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY

The content of the electronically submitted sequence listing in ASCII text file (Name: 3525.010PC02 Seq listing ST25.txt; Size: 3,634,253 bytes; and Date of Creation: Oct. 5, 2015) filed with the application is incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

The present subject matter relates, in general, to HIV and, in particular, to HIV vaccines.

BACKGROUND OF THE INVENTION

In 2013, there were approximately 2.3 million new human immunodeficiency virus (HIV) infections, over 35 million people living with HIV, and 1.6 million acquired immune deficiency syndrome (AIDS) deaths. While great progress has been made in the treatment of HIV/AIDS, all individuals living with HIV will have to be treated with anti-retroviral therapy (ART) for the rest of their lives since drug therapy is unable to clear latent viral reservoirs that exist in resting CD4+ T cells at a frequency of about 1/10⁶ cells. See, Eriksson, S. 2013. PLoS Pathog 9:e1003174.

Major strategies to purge latent HIV reservoirs are generally aimed at reactivating latent virus using histone-deacetylase (HDAC) inhibitors, however clinical studies with HDAC-inhibitors have not consistently decreased latent reservoirs. One likely reason for this lies with the inability of HIV-specific CD8+ T cells to eliminate resting CD4+ T cells.

Only a few cases have been documented where HIV-1 has been cleared from an individual with a pre-existing infection. Until an effective therapy is developed, the estimated 35 million individuals living with HIV-1 will remain on antiviral drugs to suppress a viral reservoir that has resisted all efforts at eradication.

A cure for AIDS has been elusive, but recent work suggests that stringent immunological control can clear HIV over time. Specifically, it was found that rhesus macaques (RM) vaccinated with cytomegalovirus (CMV)-based vectors expressing simian immunodeficiency virus (SIV) antigens were initially infected, but SIV was undetectable by several stringent criteria one to two years after infection. This result is even more remarkable in light of the fact that the highly virulent SIVmac239 strain used in these studies has thwarted all previous vaccine attempts. These results have expanded the current paradigm from one focused on a preventative HIV vaccine to one in which an immunotherapy for HIV/AIDS will eventually become an essential part of the fight against this pandemic. Thus, in addition to a preventative vaccine, there remains a need for an effective therapy to treat individuals living with HIV-1. Specifically, there remains a need to design, manufacture, and test prophylactic and therapeutic HIV vaccines in preparation for clinical testing.

SUMMARY OF THE INVENTION

Provided herein are HIV/SIV polypeptides comprising one or more EpiGraph antigen sequences comprising amino acid sequences corresponding to HIV/SIV Gag, Nef, Pol, Env, including full-length sequences, portions thereof, or any combination thereof. Also provided herein are HIV/SIV polypeptides comprising one or more population episensus antigen sequences comprising amino acid sequences corresponding to HIV/SIV Gag, Nef, Pol, Env, or a combination thereof. Also provided herein are one or more carriers comprising HIV/SIV polypeptides comprising one or more population episensus antigen sequences. Further provided herein are HIV/SIV polypeptides comprising one or more tailored antigen sequences comprising amino acid sequences corresponding to HIV/SIV Gag, Nef, Pol, Env, or a combination thereof. The HIV/SIV polypeptides of the present disclosure can comprise one or more HIV-1 tailored antigens, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 692-696 and SEQ ID NOs: 754-789. Also provided herein are one or more carriers comprising HIV/SIV polypeptides comprising one or more tailored antigen sequences. EpiGraph antigen sequences having SEQ ID NOs: 691-789 are provided herein.

Provided herein are HIV-1 vaccines comprising one or more carriers and one or more population episensus antigens. Also provided herein are HIV-1 vaccines comprising a vector capable of expressing a population episensus antigen and one or more tailored antigens.

In some embodiments, the HIV-1 population episensus antigen comprises epitopes from Gag, Pol, Nef, Env, Tat, Rev, Vpr, Vif, or Vpu. In some embodiments, the HIV-1 population episensus antigen is a fusion antigen comprising two or more HIV-1 population episensus antigens. In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 B clade epidemic in the United States. In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 C clade epidemic in South Africa. In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 2-clade regional epidemic in Thailand. In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 M-group global set.

In some embodiments, the HIV-1 population episensus antigen comprises epitopes from Gag. In some embodiments, the HIV-1 population episensus antigen comprises epitopes from a conserved region of HIV-1. In some embodiments, the HIV-1 population episensus antigen comprises epitopes from a conserved region of Gag, Pol, or Nef. In some embodiments, the epitopes from the conserved region of Gag are epitopes from the p24 capsid protein of Gag.

In some embodiments, the HIV-1 tailored antigen comprises epitopes from Gag, Pol, Nef, Env, Tat, Rev, Vpr, Vif, or Vpu. In some embodiments, the HIV-1 tailored antigen is central to the HIV-1 M-group global set. In some embodiments, the HIV-1 tailored antigen is central to the HIV-1 C clade epidemic in South Africa. In some embodiments, the HIV-1 tailored antigen is central to the HIV-1 B clade epidemic in the United States.

Further provided are methods of preventing or treating HIV-1 infection in a subject comprising administering an effective amount of the described HIV-1 vaccines to the subject in need thereof. Further provided are methods of designing and producing an HIV-1 vaccine for a subject comprising sequencing HIV-1 viruses in an individual, selecting vaccine antigens designed to optimally cover the diversity within a geographic area, and inserting the vaccine antigens into a vector. Also provided herein are methods of treating an HIV-1 infection in a subject comprising administering an effective amount of the disclosed vaccines to the subject in need thereof.

Also provided herein are HIV-1 vaccines comprising one or more carriers and a population episensus antigen determined using the EpiGraph approach. Further provided herein are methods of designing vaccine antigens to optimally cover the diversity within a geographic area using a vaccine antigen amino acid sequence generated using the EpiGraph method of antigen amino acid sequence selection and producing said designed vaccine antigen. Further provided herein are methods of designing and producing an HIV-1 vaccine for a subject comprising determining the amino acid sequence of HIV-1 viruses in an individual by sequencing, selecting vaccine antigens designed to optimally cover the diversity within a geographic area using a vaccine antigen amino acid sequence generated using the EpiGraph method of antigen amino acid sequence selection, and inserting the vaccine antigens into a vector.

Also provided herein are methods of inducing an effector memory T cell response comprising determining one or more EpiGraph amino acid sequences, generating a vaccine comprising the one or more EpiGraph antigen amino acid sequences and one or more carriers, and administering the vaccine to a subject in need thereof. Further provided are methods of treating HIV-1 in a subject comprising administering an effective amount of the described HIV-1 vaccines to the subject in need thereof. Also provided herein are methods of protecting from an HIV-1 infection in a subject comprising administering an effective amount of the described HIV-1 vaccines to the subject in need thereof.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1A shows a full graph for the CRF01-AE clade of the Nef protein. The rectangle is an inset shown in FIG. 1B.

FIG. 1B represents the inset from FIG. 1A. Nodes are grey dots, and represent each k-mer variant, with k=9. The edges are horizontal lines that connect epitopes whose sequences overlap by k−1 amino acids, as shown for the first two epitopes (e_(a)=VTSSNMNNA [SEQ ID NO: 790], e_(b)=TSSNMNNAD [SEQ ID NO: 791]) in the upper left. Although the topological properties of the graph do not depend on the node positions, this plot uses the vertical axis to indicate epitope frequency in the target sequence set, y=f(e), for each node. The horizontal position x(e)=1+max_(e′εp(e))x(e′), where P(e) is the set of predecessors of e, gives this plot the property that all directed edges connect from left to right. The ideal path through this graph keeps as much as possible to the largest y-values. The inset shows two paths through the nodes. The solid black line is the optimal path, and corresponds to the sequence TSSNMNNADSVWLRAQEEE [SEQ ID NO: 792] while the dashed line corresponds to TSSNMNNADCVWLRAQEEE [SEQ ID NO: 793]. The dashed line achieves higher f(e) values on 4 nodes, but the solid line has higher f(e) for 5 nodes, and Σf(e) is higher. Note there is no path that includes the highest-valued nodes for all values of x.

FIG. 2 shows nodes of an epitope graph showing the epitope k-character string, and the frequency (f) that the epitope is observed at that position in the aligned sequence set, and the cumulative best score S of consistent paths that end in that node. The nodes are arranged in columns with each column corresponding to the position t associated with the epitope. The lines connecting these nodes correspond to adjacent epitopes that are consistent. The aim is to find a consistent path through this graph that maximizes the sum of the frequency values in each node. The thicker lines show one path that leads to an optimal total score.

FIG. 3 shows that nodes from the initial solution (given by q1=ARCHSLM [SEQ ID NO: 794] as shown in FIG. 2 have their frequency values set to zero, and the cumulative scores S(t;e) are recomputed based on these new frequency nodes. This leads to an optimal solution to this complementary coverage problem: q2=DEFGKLM [SEQ ID NO: 795]. The thicker lines show one path that leads to an optimal total score.

FIGS. 4A-B show the epitope graph associated with a set of 690 US B-clade Gag protein sequences [SEQ ID NOs. 1-690], each aligned to 556 positions. The horizontal axis is the position t, and the columns of nodes indicate the different epitopes at each position. The nodes are arranged so that the most frequent at each position are at the bottom of each column. The full graph is shown in FIG. 4A; a close-up inset of the same graph is shown in FIG. 4B.

FIGS. 5A-C show that excluding rare variants decreases the coverage, but only slightly for Gag (FIG. 5A), Nef (FIG. 5B), and Pol (FIG. 5C). Coverage of polyvalent (m=2) solutions are shown as a function of minimum count f_(o) (i.e., the population frequency of the rarest epitope in the vaccine). Dashed lines correspond to coverage given by the direct sequential algorithm; the black solid lines are based on the best solutions after 100 random restarts. The vertical axis, in all three plots, is restricted to a range of 0.015.

FIGS. 6A-B show two-antigen vaccine coverage. Comparisons illustrating the average epitope coverage per sequence of the contemporary B clade sequences isolated in the United States, which was considered as a hypothetical target population for a therapeutic vaccine. To illustrate potential T-cell epitope (PTE) coverage using a pair of natural within-clade sequences as vaccine antigens, 5000 randomly selected pairs of natural B clade sequences (gray) were evaluated as potential vaccines, and the distribution of average coverage of the 189 contemporary B clade US sequences is shown in the grey histogram. This is compared to the average coverage provided by a 2 antigen set of M group EpiGraphs (M database), a two antigen set of global B clade EpiGraphs (B database), and a US B clade tailored vaccine where the 2 best matches from a set of 6 representative EpiGraphs for manufacture were chosen as a “tailored” match for each of the 189 natural B clade US sequences. FIG. 6A shows the comparison for the full Gag protein. FIG. 6B shows comparisons for only the conserved p24 region.

FIG. 7 shows mapping potential epitope coverage spanning the HIV proteome.

FIG. 8 shows the average potential Gag epitope coverage of the HIV-1 B clade U.S. sequences by different vaccine antigens. FIG. 8A shows the average Gag epitope coverage by single vaccine antigens, and FIG. 8B shows the average Gag epitope coverage by pairs of vaccine antigens.

FIGS. 9A-C show that EpiGraph designed synthetic HIV antigens are expressed as full-length proteins. HeLa cells were transfected with expression plasmids encoding: fusion proteins of gag and nef for HIV or SIV (HIV M group EpiGraph 1 [SEQ ID NO: 705] and HIV M group EpiGraph 2 [SEQ ID NO: 707], SIVmac239 full length proteins, SIV variants hybrid proteins [SEQ ID NO: 713], and SIV conserved portions of gag and nef [SEQ ID NO: 714]), as represented in FIG. 9A; polymerase proteins of HIV or SIV (SIVmac239 full-length (FL), SIV variants hybrid proteins [SEQ ID NO: 715], (HIV M group EpiGraph 1[SEQ ID NO: 709] and HIV M group EpiGraph 2 [SEQ ID NO: 711], and SIV conserved portions of pol [SEQ ID NO: 716]), as represented in FIG. 9B; and fusion proteins of gag and nef for HIV (clade B EpiGraph 1 gag/nef [SEQ ID NO: 701]) and clade B EpiGraph 2 gag/nef [SEQ ID NO: 702]) and polymerase proteins for HIV (clade M epi 1 pol [SEQ ID NO: 709]); clade B EpiGraph 1 pol [SEQ ID NO: 703]); clade B EpiGraph 2 pol [SEQ ID NO: 704]), as represented in FIG. 9C. All gag/nef constructs include a carboxy-terminal V5 tag (FIG. 9A), and all pol constructs include a carboxy-terminal HA tag (FIGS. 9B and 9C). For FIG. 9B, 1 and 2 indicate multiple clones of each construct. Lysates were harvested at 48 hours post-transfection and immunoblotted using V5 (FIG. 9A) or HA (FIGS. 9B and 9C) antibodies. The observed molecular weight of the proteins is consistent with the predicted molecular weight for each of the constructs.

FIGS. 10A-B show that EpiGraph designed synthetic antigens are expressed by CMV vectors. As represented in FIG. 10A, RhCMV strain 68-1 expressing SIVmac 239 polymerase full-length (FL), a hybrid of SIV polymerases from different SIV variants [SEQ ID NO: 715], a synthetic polymerase gene based on global M group HIV EpiGraph 1 [SEQ ID NO: 709] or global M group HIV EpiGraph 2 [SEQ ID NO: 711] were constructed by BAC mutagenesis and reconstituted in telomerized Rhesus fibroblasts. As represented in FIG. 10B, RhCMV strain 68-1 expressing SIVmac239 conserved regions of the polymerase constructs [SEQ ID NO: 716] were also constructed by BAC mutagenesis and reconstituted in telomerized Rhesus fibroblasts. In all vectors, antigen expression is driven by the endogenous viral Rh107 promoter. Cell pellets were harvested at full CPE and immunoblotted for the HA tag expressed at the carboxy-terminus of each protein. For pol conserved, two independent clones (2.1 and 2.2) are shown in FIG. 10B.

FIGS. 11A-C shows conserved regions within: Nef (FIG. 11A), Gag (FIG. 11B), and Pol (FIG. 11C). defined based on the potential for potential T-cell epitope (PTE) coverage by a bivalent (i.e., 2 antigen) vaccine.

FIG. 12 illustrates the average Epigraph coverage of each of the conserved regions by different vaccine antigens, compared to the more variable sections of the proteins.

DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

Various terms relating to aspects of the description are used throughout the specification and claims. Such terms are to be given their ordinary meaning in the art unless otherwise indicated. Other specifically defined terms are to be construed in a manner consistent with the definitions provided herein.

As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. Thus, for example, reference to “a cell” includes a combination of two or more cells, and the like.

The term “about” as used herein when referring to a measurable value such as an amount, a temporal duration, and the like, is meant to encompass variations of up to ±20% from the specified value, as such variations are appropriate to perform the disclosed methods. Unless otherwise indicated, all numbers expressing quantities of ingredients, properties such as molecular weight, reaction conditions, and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

As employed above and throughout the disclosure the term “effective amount” refers to an amount effective, at dosages, and for periods of time necessary, to achieve the desired result with respect to the treatment of the relevant disorder, condition, or side effect. It will be appreciated that the effective amount of components of the present invention will vary from patient to patient not only with the particular vaccine, component or composition selected, the route of administration, and the ability of the components to elicit a desired result in the individual, but also with factors such as the disease state or severity of the condition to be alleviated, hormone levels, age, sex, weight of the individual, the state of being of the patient, and the severity of the pathological condition being treated, concurrent medication or special diets then being followed by the particular patient, and other factors which those skilled in the art will recognize, with the appropriate dosage being at the discretion of the attending physician. Dosage regimes may be adjusted to provide the improved therapeutic response. An effective amount is also one in which any toxic or detrimental effects of the components are outweighed by the therapeutically beneficial effects.

The term “administering” means either directly administering a compound or composition of the present invention, or administering a prodrug, derivative or analog which will form an equivalent amount of the active compound or substance within the body.

The terms “subject,” “individual,” and “patient” are used interchangeably herein, and refer to an animal, for example a human, to whom treatment, including prophylactic treatment, with the pharmaceutical composition according to the present invention, is provided. The term “subject” as used herein refers to human and non-human animals. The terms “non-human animals” and “non-human mammals” are used interchangeably herein and include all vertebrates, e.g., mammals, such as non-human primates, (particularly higher primates), sheep, dog, rodent, (e.g., mouse or rat), guinea pig, goat, pig, cat, rabbits, cows, horses and non-mammals such as reptiles, amphibians, chickens, and turkeys.

The term “cocktail” refers to a set of antigens intended to be delivered in combination to a patient.

The term “episensus” refers to an epitope based consensus sequence. It is a sequence whose epitopes match, as closely as possible, the epitopes in a reference set of natural sequences. The terms “epitope” and “potential epitope” refer to a sequence of k characters (typically k is in the range of 8-12), often in the context of a k-character subsequence of a much longer natural or vaccine antigen sequence. T cells can recognize such peptides in an immune response.

The term “EpiGraph” refers to a new computational strategy developed to create sequences that provide an optimal episensus sequence, or set of sequences that combined provide optimal coverage of a population of diverse viral sequences.

The terms “EpiGraph sequence” or “Episensus antigen” refer to the vaccine inserts designed based on the EpiGraph algorithm.

The term “population episensus antigen” refers to a sequence derived with the EpiGraph algorithm, which are “central” to a population of HIV sequences. The population could be a specific HIV clade, cluster of sequences derived using our Tailored epitope based clustering algorithm, or the global epidemic. “Central” is defined in terms of sharing potential epitopes, so it is a computationally-derived sequence that provides the maximal average epitope coverage of the population.

EpiGraph sequences can be used as a solution for a prophylactic, preventive vaccine, or can be adopted as part of more complex strategies for the design of therapeutic vaccines that would be tailored to match individual infections. The term “tailored vaccine set” refers to a set of vaccine antigen sequences designed for manufacture, from which a subset antigens could be selected to best match a patient's virus for delivery as a therapeutic vaccine.

The term “tailored antigen” or “tailored episensus antigen” refers to one or more amino acid sequences from the “tailored vaccine set” that would be specifically selected based on a best match to a patient's infecting HIV-1 strain. for delivery to that patient as a therapeutic vaccine.

As used herein, the terms “treatment” or “therapy” (as well as different forms thereof, including curative or palliative) refer to treatment of an infected person. As used herein, the term “treating” includes alleviating or reducing at least one adverse or negative effect or symptom of a condition, disease or disorder. This condition, disease or disorder can be HIV infection.

As used herein, the terms “prevention” or “prophylaxis” refer to preventing a subject from becoming infected with, or reducing the risk of a subject from becoming infected with, or halting transmission of, or the reducing the risk of transmission of, for example, HIV, SIV, or a related virus.

“Pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem complications commensurate with a reasonable benefit/risk ratio.

Vectors that can be used include, but are not limited to, plasmids, bacterial vectors, and viral vectors. Viral vectors include cytomegalovirus (CMV) vectors. An advantage of these CMV vectors for use in therapeutic vaccine delivery is that they create a new CD8+ T cell epitope paradigm and induce more potent and enduring responses. It has been shown in animal models that vaccines based on these viral vectors can clear viral infections (Hansen, S. G. 2013. Science 340:1237874), and so these approaches have promise for a therapeutic vaccine, a setting in which tailored vaccines can be useful.

Other viral vectors can include poxvirus (vaccinia), including vaccinia Ankara and canarypox; adenoviruses, including adenovirus type 5 (Ad5); rubella; sendai virus; rhabdovirus; alphaviruses; and adeno-associated viruses. Alternatively, EpiGraph vaccine antigens could be delivered as DNA, RNA or protein components of a vaccine. As this is an antigen design strategy, EpiGraph-designed vaccine antigens would be compatible with essentially any mode of vaccine antigen delivery.

In certain embodiments, vaccines designed using EpiGraph amino acid sequences have a single antigen. In certain embodiments, vaccines designed using EpiGraph amino acid sequences have sets of antigens.

In certain embodiments, the EpiGraph antigen sequences can be used in a prophylactic vaccine setting.

In certain embodiments, the EpiGraph strategy can be used to make sequences that fully optimize epitope coverage for a prophylactic vaccine. This could be one sequence, or several sequences designed to complement each other as a preventative vaccine. EpiGraph vaccines could be used in any vector, including, but not limited to, plasmids, bacterial vectors, and viral vectors.

In the EpiGraph algorithm, the natural sequences are characterized by a large graph of nodes, each node corresponding to an epitope that appears in the natural sequences. Directed edges connect two nodes when the corresponding two epitope strings are “consistent”, meaning the last k−1 characters in the first string agree with the first k−1 characters in the second string (e.g., “EPTAPPAEPTAP” [SEQ ID NO: 796] and “PTAPPAEPTAPP” [SEQ ID NO: 797] are consistent k=12-mers). If two strings are consistent, then a string of length k+1 (e.g., “EPTAPPAEPTAPP”) [SEQ ID NO: 798] contains both epitopes. More generally, a path through this graph of nodes and edges corresponds to a single string that contains k-mer substrings corresponding to each of the nodes in the graph. Each node is weighted according to how many sequences in the reference set exhibit a substring corresponding to that node. The EpiGraph algorithm uses a dynamic programming scheme to find the path through this full graph that maximizes the sum of these weights, and therefore provides the greatest coverage.

Certain embodiments provided include an HIV-1 vaccine comprising a vector and a population episensus antigen, or combination of optimized EpiGraph antigens designed to be used as a set. There are many different vectors that could be used for the vaccine. For example, one type of vector that can be used is a viral vector. These viral vectors can include a human cytomegalovirus (HCMV), a poxvirus, adenoviruses, rubella, sendai virus, rhabdovirus, alphavirus or adeno-associated virus. The vaccine could also be delivered as a gene encoding the EpiGraph protein, either using DNA, RNA, or included as an expressed protein or part of a protein.

In certain embodiments, EpiGraph antigen of the disclosed vaccines can be derived from the HIV-1 Gag protein. In another embodiment, the HIV-1 Gag protein has been inactivated by eliminating a myristoylation sequence in the N-terminus of the HIV-1 Gag protein. In another embodiment, the EpiGraph antigen can be derived from the HIV-1 Pol or Nef protein, or indeed any other HIV protein, including Env, Tat, Rev, Vif, or Vpu.

In certain embodiments, the population episensus antigen can be determined using the EpiGraph approach. This population episensus antigen could then be used to create a vaccine. Alternatively, EpiGraphs could be designed as a combination of sequences designed to be used as a set. HIV-1 can be split into clades based on geographic location. In one embodiment, the population episensus antigen is central to the HIV-1 B clade epidemic in the United States in the disclosed vaccines. In another embodiment, the population episensus antigen is central to the HIV-1 C clade epidemic in South Africa in the disclosed vaccines. In another embodiment, the population episensus antigen is central to the HIV-1 2-clade regional epidemic in Thailand in the disclosed vaccines. In another embodiment, the population episensus antigen is central to the HIV-1 M-group global set in the disclosed vaccines.

In some embodiments, the HIV-1 population episensus antigen comprises epitopes from Gag, Pol, Nef, Env, Tat, Rev, Vpr, Vif, or Vpu. In some embodiments, the HIV-1 population episensus antigen comprises epitopes from Gag and comprises the amino acid sequence of SEQ ID NO: 691, SEQ ID NO: 697, SEQ ID NO: 698, SEQ ID NO: 699, or SEQ ID NO: 700. In some embodiments, the HIV-1 population episensus antigen comprises epitopes from a conserved region of HIV-1. In some embodiments, the HIV-1 population episensus antigen comprises epitopes from a conserved region of Gag, Pol, or Nef. In some embodiments, the epitopes from the conserved region of Gag are epitopes from the p24 capsid protein of Gag.

In some embodiments, the HIV-1 population episensus antigen is a fusion antigen comprising two or more HIV-1 population episensus antigens. In some embodiments, the fusion antigen comprises a HIV-1 population episensus antigen comprising Gag epitopes and a HIV-1 population episensus antigen comprising Nef epitopes. In some embodiments, the fusion antigen comprises a HIV-1 population episensus antigen comprising epitopes from a conserved region of Gag and a HIV-1 population episensus antigen comprising epitopes from a conserved region of Nef. In some embodiments, the epitopes of the fusion antigen from the conserved region of Gag are epitopes from the p24 capsid protein of Gag.

In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 B clade epidemic in the United States. In some embodiments, the HIV-1 B clade population episensus antigen comprises Gag epitopes and comprises the amino acid sequence of SEQ ID NO: 730, SEQ ID NO: 732, or SEQ ID NO: 778. In some embodiments, the HIV-1 B clade population episensus antigen comprises epitopes from the p24 capsid protein of Gag and comprises the amino acid sequence of SEQ ID NO: 731, SEQ ID NO: 733, or SEQ ID NO: 779. In some embodiments, the HIV-1 B clade population episensus antigen comprises Nef epitopes and comprises the amino acid sequence of SEQ ID NO: 734 or SEQ ID NO: 736. In some embodiments, the HIV-1 B clade population episensus antigen comprises epitopes from a conserved region of Nef and comprises the amino acid sequence of SEQ ID NO: 735 or SEQ ID NO: 737. In some embodiments, the HIV-1 B clade population episensus antigen comprises Pol epitopes and comprises the amino acid sequence of SEQ ID NO: 703, SEQ ID NO: 704, SEQ ID NO: 738, or SEQ ID NO: 740. In some embodiments, the HIV-1 B clade population episensus antigen comprises epitopes from a conserved region of Pol and comprises the amino acid sequence of SEQ ID NO: 739 or SEQ ID NO: 741. In some embodiments, the HIV-1 B clade population episensus antigen is a fusion antigen comprising (1) a HIV-1 B clade population episensus antigen comprising Gag epitopes and (2) a HIV-1 B clade population episensus antigen comprising Nef epitopes, wherein the fusion antigen comprises the amino acid sequence of SEQ ID NO: 701 or SEQ ID NO: 702. In some embodiments, the HIV-1 B clade population episensus antigen is a fusion antigen comprising (1) a HIV-1 B clade population episensus antigen comprising epitopes from the p24 capsid protein of Gag and (2) a HIV-1 B clade population episensus antigen comprising epitopes from a conserved region of Nef.

In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 C clade epidemic in South Africa. In some embodiments, the HIV-1 C clade population episensus antigen comprises Gag epitopes and comprises the amino acid sequence of SEQ ID NO: 742, SEQ ID NO: 744, or SEQ ID NO: 766. In some embodiments, the HIV-1 C clade population episensus antigen comprises epitopes from the p24 capsid protein of Gag and comprises the amino acid sequence of SEQ ID NO: 743, SEQ ID NO: 745, or SEQ ID NO: 767. In some embodiments, the HIV-1 C clade population episensus antigen comprises Nef epitopes and comprises the amino acid sequence of SEQ ID NO: 746 or SEQ ID NO: 748. In some embodiments, the HIV-1 C clade population episensus antigen comprises epitopes from a conserved region of Nef and comprises the amino acid sequence of SEQ ID NO: 747 or SEQ ID NO: 749. In some embodiments, the HIV-1 C clade population episensus antigen comprises Pol epitopes and comprises the amino acid sequence of SEQ ID NO: 750 or SEQ ID NO: 752. In some embodiments, the HIV-1 C clade population episensus antigen comprises epitopes from a conserved region of Pol and comprises the amino acid sequence of SEQ ID NO: 751 or SEQ ID NO: 753. In some embodiments, the HIV-1 C clade population episensus antigen is a fusion antigen comprising (1) a HIV-1 C clade population episensus antigen comprising Gag epitopes and (2) a HIV-1 C clade population episensus antigen comprising Nef epitopes. In some embodiments, the HIV-1 C clade population episensus antigen is a fusion antigen comprising (1) a HIV-1 C clade population episensus antigen comprising epitopes from the p24 capsid protein of Gag and (2) a HIV-1 C clade population episensus antigen comprising epitopes from a conserved region of Nef.

In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 2-clade regional epidemic in Thailand. In some embodiments, the HIV-1 2-clade population episensus antigen comprises Gag epitopes. In some embodiments, the HIV-1 2-clade population episensus antigen comprises epitopes from the p24 capsid protein of Gag. In some embodiments, the HIV-1 2-clade population episensus antigen comprises Nef epitopes. In some embodiments, the HIV-1 2-clade population episensus antigen comprises epitopes from a conserved region of Nef. In some embodiments, the HIV-1 2-clade population episensus antigen comprises Pol epitopes. In some embodiments, the HIV-1 2-clade population episensus antigen comprises epitopes from a conserved region of Pol. In some embodiments, the HIV-1 2-clade population episensus antigen is a fusion antigen comprising (1) a HIV-1 2-clade population episensus antigen comprising Gag epitopes and (2) a HIV-1 2-clade population episensus antigen comprising Nef epitopes. In some embodiments, the HIV-1 2-clade population episensus antigen is a fusion antigen comprising (1) a HIV-1 2-clade population episensus antigen comprising epitopes from the p24 capsid protein of Gag and (2) a HIV-1 2-clade population episensus antigen comprising epitopes from a conserved region of Nef.

In some embodiments, the HIV-1 population episensus antigen is central to the HIV-1 M-group global set. In some embodiments, the HIV-1 M-group population episensus antigen comprises Gag epitopes and comprises the amino acid sequence of SEQ ID NO: 718, SEQ ID NO: 720, or SEQ ID NO: 754. In some embodiments, the HIV-1 M-group population episensus antigen comprises epitopes from the p24 capsid protein of Gag and comprises the amino acid sequence of SEQ ID NO: 719, SEQ ID NO: 721, or SEQ ID NO: 755. In some embodiments, the HIV-1 M-group population episensus antigen comprises Nef epitopes and comprises the amino acid sequence of SEQ ID NO: 722 or SEQ ID NO: 724. In some embodiments, the HIV-1 M-group population episensus antigen comprises epitopes from a conserved region of Nef and comprises the amino acid sequence of SEQ ID NO: 723 or SEQ ID NO: 725. In some embodiments, the HIV-1 M-group population episensus antigen is a fusion antigen comprising (1) a HIV-1 M-group population episensus antigen comprising Gag epitopes and (2) a HIV-1 M-group population episensus antigen comprising Nef epitopes, wherein the fusion antigen comprises the amino acid sequence of SEQ ID NO: 705 or SEQ ID NO: 707. In some embodiments, the HIV-1 M-group population episensus antigen is a fusion antigen comprising (1) a HIV-1 M-group population episensus antigen comprising epitopes from the p24 capsid protein of Gag and (2) a HIV-1 M-group population episensus antigen comprising epitopes from a conserved region of Nef, wherein the fusion antigen comprises the amino acid sequence of SEQ ID NO: 706 or SEQ ID NO: 708. In some embodiments, the HIV-1 M-group population episensus antigen comprises Pol epitopes and comprises the amino acid sequence of SEQ ID NO: 709, SEQ ID NO: 711, SEQ ID NO: 726, or SEQ ID NO: 728. In some embodiments, the HIV-1 M-group population episensus antigen comprises epitopes from a conserved region of Pol and comprises the amino acid sequence of SEQ ID NO: 710, SEQ ID NO: 712, SEQ ID NO: 727, or SEQ ID NO: 729.

In certain embodiments, the disclosed vaccines can further comprise a HCMV vector comprising a HCMV backbone and a tailored antigen selected to be a best match natural HIV-1 strain. In another embodiment, the disclosed vaccines can further comprise a HCMV vector comprising a HCMV backbone and a tailored antigen selected to be a best match different HIV-1 strain.

In some embodiments, the HIV-1 tailored antigen comprises epitopes from Gag, Pol, Nef, Env, Tat, Rev, Vpr, Vif, or Vpu. In some embodiments, the tailored antigen comprises a sequence selected from: SEQ ID NOs. 692-696; SEQ ID NOs. 756-765; SEQ ID NOs. 769-777; or SEQ ID NOs. 780-789.

In some embodiments, the HIV-1 tailored antigen comprises epitopes from Gag and comprises the amino acid sequence of SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695, or SEQ ID NO: 696.

In some embodiments, the HIV-1 tailored antigen is central to the HIV-1 M-group global set. In some embodiments, the HIV-1 M-group tailored antigen comprises Gag epitopes and comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 756-765. In some embodiments, the HIV-1 M-group tailored antigen comprises epitopes from the p24 capsid protein of Gag and comprises the amino acid sequence of SEQ ID NO: 757, SEQ ID NO: 759, SEQ ID NO: 761, SEQ ID NO: 763, or SEQ ID NO: 765.

In some embodiments, the HIV-1 tailored antigen is central to the HIV-1 C clade epidemic in South Africa. In some embodiments, the HIV-1 C clade tailored antigen comprises Gag epitopes and comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 768-777. In some embodiments, the HIV-1 C clade tailored antigen comprises epitopes from the p24 capsid protein of Gag and comprises the amino acid sequence of SEQ ID NO: 769, SEQ ID NO: 771, SEQ ID NO: 773, SEQ ID NO: 775, or SEQ ID NO: 777.

In some embodiments, the HIV-1 tailored antigen is central to the HIV-1 B clade epidemic in the United States. In some embodiments, the HIV-1 B clade tailored antigen comprises Gag epitopes and comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 780-789. In some embodiments, the HIV-1 B clade tailored antigen comprises epitopes from the p24 capsid protein of Gag and comprises the amino acid sequence of SEQ ID NO: 781, SEQ ID NO: 783, SEQ ID NO: 785, SEQ ID NO: 787, or SEQ ID NO: 789.

In certain embodiments, methods of treating HIV-1 in a subject comprising administering an effective amount of one of the disclosed vaccines to the subject in need thereof is provided. In another embodiment, the selecting vaccine antigens to optimally cover the diversity within a geographical area uses a vaccine antigen sequence generated using the EpiGraph method of antigen sequence selection.

Embodiments of the present invention include methods of treating an HIV-1 infection in a subject comprising administering an effective amount of any of these disclosed vaccines to the subject in need thereof.

Embodiments of the present invention also include methods of inducing an effector memory T cell response comprising determining one or more EpiGraph sequences, generating a vaccine comprising the one or more EpiGraph amino sequences and a vector; and, administering the vaccine to a subject in need thereof. In another embodiment, methods are provided of inducing an effector memory T cell response wherein the one or more EpiGraph amino sequences comprises SEQ ID NO: 697, SEQ ID NO: 698, SEQ ID NO: 699, or SEQ ID NO: 700. This vaccine can be a prophylactic or therapeutic vaccine.

Recent breakthroughs in HIV vaccine research include the concept of an effector memory T cell (TEM)-inducing vaccine to prevent HIV infection. Unlike central memory T cells (TCM) induced by traditional vaccine approaches, TEM are persistently maintained in lymphoid tissues and extralymphoid effector sites and are immediately ready to mediate anti-viral effector function, thus providing a constant immune shield at the portals of viral entry and sites of viral reactivation. The most qualified vector system to induce and indefinitely maintain TEM is derived from CMV. Presumably due to continuous, low-level reactivation and/or gene expression in persistently infected cells, CMV maintains just the right amount of persistent, low level immune stimulation required for TEM maintenance without triggering T cell exhaustion.

In certain embodiments, the tailored antigen cocktail can be used in a therapeutic vaccine setting. For example, the tailored vaccine can use a k-means clustering strategy to a defined set of 6-10 sequences that provide very good coverage of epitopes in a population of people that are infected with a highly variable pathogen, such as HIV. The virus infecting a subject can be sequenced and 2 or 3 tailored vaccines will be delivered that best cover the infecting virus. Epitope coverage will be optimized while epitope mismatches will be will be minimized between the vaccine and the infecting strain.

Certain embodiments provided include an HIV-1 vaccine comprising a HCMV backbone vector, which lacks certain CMV gene regions, and a population episensus antigen. In certain embodiments, the HCMV backbone can lack the UL131A-128 gene region. Certain embodiments can also include deletion of the tegument protein pp71 (UL82) gene. (U.S. Patent Application Publication Nos. 2014-0141038; 2008-0199493; 2013-0142823: and International Application Publication No. WO/2014/138209).

In certain embodiments, the present disclosure provides vaccines which can comprise a second tailored antigen sequence. In certain embodiments, the vaccines can comprise a second, a third, or more tailored antigen sequences. The episensus antigen can comprise the amino acid sequence of SEQ ID NO: 691. The tailored antigen can comprise the amino acid sequence of SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695 or SEQ ID NO: 696. In one embodiment, the first tailored antigen sequence and the second tailored antigen sequence comprise two of the amino acid sequences of SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695 and SEQ ID NO: 696. In another embodiment, the first tailored antigen sequence, the second tailored antigen sequence and the third tailored antigen sequence comprises three of the amino acid sequences of SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695 and SEQ ID NO: 696.

Also provided are tailored antigen sequences that can comprise SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695 or SEQ ID NO: 696.

In certain embodiments, the disclosed vaccines can further comprise a HCMV vector comprising a HCMV backbone and a tailored antigen selected to be a best match natural HIV-1 strain. In another embodiment, the disclosed vaccines can further comprise a HCMV vector comprising a HCMV backbone and a tailored antigen selected to be a best match different (and distant) HIV-1 strain.

In certain embodiments, the episensus antigen and the tailored antigen of the disclosed vaccines can be derived from the HIV-1 Gag protein. In another embodiment, the HIV-1 Gag protein has been inactivated by eliminating a myristoylation sequence in the N-terminus of the HIV-1 Gag protein. In another embodiment, the episensus antigen and the tailored antigen can be derived from the HIV-1 Pol or Nef protein.

In certain embodiments, methods of treating HIV-1 in a subject comprising administering an effective amount of one of the disclosed vaccines to the subject in need thereof is provided. In another embodiment, methods of designing and producing an HIV-1 vaccine for a subject comprising sequencing HIV-1 viruses in an individual, selecting vaccine antigens designed to optimally cover the diversity within a geographic area, and, inserting the vaccine antigens into a vector are provided.

Certain embodiments include methods of treating an HIV-1 infection in a subject comprising administering an effective amount of any of this disclosed vaccines to the subject in need thereof.

The following examples are provided to describe the embodiments described herein with greater detail. They are intended to illustrate, not to limit, the embodiments.

All documents, patent, and patent applications cited herein are hereby incorporated by reference, and may be employed in the practice of the invention.

EXAMPLES Example 1: Graphical Model for Optimal Epitope Coverage of Aligned Sequences

The existence of a set S={s₁, s₂, . . . , s_(N)} of N aligned sequences was presumed. Each sequence is a string of alphabetic characters (corresponding to the twenty amino acids, possibly in addition to a few special characters corresponding to gaps, unknowns, and such). Since the sequences are aligned, all have the same length T, and the positions t=1 . . . T are well-defined from sequence to sequence; s_(n) [t] will be written as the t'th character in the n'th sequence. It is useful to introduce the notation s[t:u] for the subsequence of s that begins at position t and ends at position u.

A potential epitope was defined as a short sequence of k characters, typically 8 to 12. Epitopes of interest are subsequences of the sequences in S. Indeed, a sequence s can be thought of as a list of epitopes: e₁, . . . , e_(T−k+1), with e_(t)=s[t:t+k−1]. Note, however, that for a list of epitopes to be associated with a sequence, it is necessary that the epitopes be consistent. Specifically, it is required for any adjacent pair e_(t) and e_(t+1) that the last k−1 characters of e_(t) agree with the first k−1 characters of e_(t+1); that is: e_(t) [2:k]=e_(t+1) [1:k−1].

For each epitope e, a frequency f(e) can be associated which counts the fraction of sequences in S for which the epitope appears.

For the general cocktail problem, a set of artificial sequences Q={q₁, . . . , q_(M)} is sought whose epitopes have useful properties. Let E_(Q) be the set of all epitopes that appear in Q; that is:

E _(Q) ={e|e=q _(m) [t:t+k−1] for some choice of m,t}.

In particular, those epitopes were designed to cover as much of the sequence set S as possible. The goal was to maximize the coverage score

Σf(e) where the sum is over e

E _(Q)

With M<N, and typically M

N, all the epitopes cannot in general be covered.

For the aligned sequence problem, some simplifications can be made. In this case, each position in the alignment was treated as a different (but not independent) problem. The frequency function now depended on position: f (t, e) was the number of sequences in S for which e is the k-mer subsequence that began at position t; thus,

f(t,e)=ΣI(e=s _(n) [t:t+k−1])

where I is the indicator function: it is one if its argument is true, and is zero otherwise. The set E_(Q) can also be partitioned according to position in sequences q. Thus:

E _(Q) [t]={e|e=q _(m) [t:t+k−1] for some m}

This enables a production of a coverage measure for each position c[t]=Σ_(eεEQ[t])f(t,e), from which an overall coverage is given by c=Σ_(t) c[t].

For the trivial special case k=1, the epitopes were just the amino acid characters. But even though the problem was trivial to solve, the solution was still useful. For M=1, the best solution is given by the consensus sequence, with q₁ [t] chosen to be the amino acid that is most common at position t. For M=2, one can optimize the coverage by taking q₂ [t] to be the second-most-common amino acid at position t. And so on for larger M.

For k>1, the problem becomes nontrivial because the epitopes overlap, and so each c[t] can no longer be optimized independently. The M=1 case was called the “episensus” problem because it is like the consensus, except that it is a consensus of epitopes that was sought.

In one example, the consensus and the k=3 episensus disagree: The consensus used the most popular letter in each position. The episensus used the most popular “epitope”, where we call a potential epitope a string of 3 characters. Table 1 expands this example to illustrate overlapping epitope strings.

TABLE 1 Shown are six sequences and their associated k = 3 epitopes. The bottom line shows the consensus  sequences (formed from the most common character at each position) and the most common epitopes at  each position. But these epitopes (in particular, EFG and CHS) are inconsistent with each other, so they cannot be combined into a  single episensus solution. In this case, the best episensus score is given (though not uniquely)  by the sequence ARCHSLM [SEQ ID NO: 794], which covers 1 + 1 + 2 + 2+  3 = 9 out of 30 possible   epitopes in the sequences. The consensus, ARCGSLM [SEQ ID NO: 799], covers 1 + 1 + 1 + 1 +   3 the frequency of the most popular epitopes at each position: in this case, that gives 2 + 2 + 2 + 2 + 3 = 11. Sequences Toy Epitopes [SEQ ID NO: 800] ARCGSPM ARC RCG CGS GSP SPM [SEQ ID NO: 801] ARYGSNM ARY RYG YGS GSN SNM [SEQ ID NO: 802] AYCHSLM AYC YCH CHS HSL SLM [SEQ ID NO: 803] YRCHSLM YRC RCH CHS HSL SLM [SEQ ID NO: 804] DEFGSLM DEF EFG FGS GSL SLM [SEQ ID NO: 805] DEFGKLM DEF EFG FGK TKL KLM

Solving the Episensus Problem.

The M=1 case was addressed first, in which a single sequence q whose epitopes optimally cover the epitopes in an unaligned sequence list S was sought. The EpiGraph algorithm under appropriate assumptions achieves the optimal solution. In the comparisons, the consensus algorithm was also considered (very simple and fast) and the optimization by genetic algorithm (very slow) as described in (Fisher, Nat Med. 2007 January; 13(1):100-6, incorporated herein by reference). The EpiGraph algorithm is illustrated in FIGS. 1A and 1B.

Later the more general cocktail of vaccines problem was considered, with M>1 and shown how the episensus algorithm were modified for this more general problem.

FIG. 2 shows nodes of an epitope graph, where each node includes the epitope k-character string, and the frequency (f) that the epitope is observed at that position in the aligned sequence set, and the cumulative best score S of consistent paths that end on that node. The nodes were arranged in columns with each column corresponding to the position t associated with the epitope. The lines connecting these nodes correspond to adjacent epitopes that were consistent. The aim is to and a consistent path through this graph that maximized the sum of the frequency values in each node.

The thicker lines in FIG. 2 show one path that leads to an optimal total score. There will always be at least one such path, but it may not be unique.

The cumulative score S(t, e) was defined as the highest score achievable starting at t=1 and finishing at the epitope e at position t. It was observed that S(t=1, e)=f(e) and that values for t>1 can be computed recursively:

S(t,e)=f(e)+max_(e′) S(t−1,e′), with e′

E(t−1,e)

where E (t−1, e) is the set of epitopes at position t−1 that are consistent with e, which is at position t. Having computed this cumulative score for all the epitopes, the total score was found for the best path as the maximum score on the last column: S_(max)=max_(e) S(T−k+1, e). Furthermore, it can be worked backwards from this maximum to find the optimal path:

e* _(T−k+1)=argmax_(e) S(T−k+1,e)

e* _(t−1)=argmax_(e′) S(t−1,e′) with e′

E(t−1,e* _(t))

The sequence e*₁, e*₂, . . . , e*_(T−k) defined the highest-scoring consistent sequence of epitopes. The episensus string q is obtained by taking the first character from each epitope: q[t]=e_(t)*[1], and finishing off with the last epitope: q[T−k:T]=e*_(T−k)[1:k].

The argmax operator may not have a unique value; if it does not, then there will be multiple solutions to the episensus problem, all of which are optimal in the sense of coverage.

Gaps.

In order to align sequences, insertions and deletions have to be dealt with, and this introduces gaps into the aligned sequences. For example, the sequences ARCCDEGH [SEQ ID NO: 806] and ARCDEFGH [SEQ ID NO: 807] were better aligned as ARCCDE-GH [SEQ ID NO: 808] and ARC-DEFGH [SEQ ID NO: 809]. Placeholder epitopes were developed to deal with these gaps in the EpiGraph algorithm.

Placeholder Epitopes:

The k=3 epitopes were ARC, RCC, CCD, CDE, DEG, EGH and ABC, BCD, CDE, DEF, EFG, FGH respectively; but when epitopes were aligned by first column gaps need to be introduced in that list: ABC, BCC, CCD, CDE, DEG, EGH, -GH and ABC, BCD, CDE, -DE, DEF, EFG, FGH. The strings -GH and -DE were placeholder epitopes. Placeholders are not counted in the covering function; that is: f(t; −XY)=0. But they were still useful because they can be used to define consistency of adjacent epitopes.

For ungapped sequences, two adjacent epitopes were considered consistent if the last k−1 characters of the first epitope agree with the first k−1 characters of the second epitope. Thus ABC and BCD are consistent, but BCC and CDE are not. When gaps are introduced, then a pair is consistent if the second epitope begins with a gap character is considered, and the remaining k−1 characters match the last k−1 characters of the last epitope. Thus, CDE and -DE are consistent.

For the drop-in-place algorithm, a “substrate” sequence that is generally taken to be the consensus sequence was used. Then, all the epitopes at all the positions were taken and sorted according to how often they appear. Starting from the least frequent epitope, each epitope was dropped onto the substrate by replacing the characters in the substrate at the positions [t:t+k−1] with the characters in the epitope. When the most frequently occurring epitope was dropped onto the substrate, a string was used as the episensus solution q. Since the most frequent epitopes overwrite the rarer epitopes, higher epitope coverage was achieved. And since a single sequence q is always updated, the final solution will be composed of consistent epitopes.

The algorithm may not be fully deterministic because some epitopes might have identical frequencies, and if their positions are overlapping, then the final result may depend on what order they are dropped. On implementation, the order that the sort algorithm gives is taken, but there is an opportunity to randomize those orders and to make multiple runs of the algorithm, with some runs possibly giving higher scores.

Heuristic “Drop-in-Place” Algorithm.

In this algorithm, a “substrate” sequence was taken to be the consensus sequence, but the choice of substrate rarely makes any difference. All the epitopes at all the positions were taken and sorted according to how often they appear. Starting from the least frequent epitope.

Each epitope was “dropped” onto the substrate by replacing the characters in the substrate at the positions [t:t+k−1] with the characters in the epitope. When the most frequently occurring epitope was finally dropped onto the substrate, there was a string that was used as episensus solution q. Since the most frequent epitopes overwrite the rarer epitopes, a high epitope coverage was achieved. Since a single sequence q was always being updated, the final solution was composed of consistent epitopes.

The algorithm may not be fully deterministic because some epitopes might have identical frequencies, and if their positions are overlapping, the n the final result may depend on what order they are dropped. In implementation, the order that the sort algorithm gives is taken, but there is an opportunity to randomize those orders and to make multiple runs of the algorithm, with some runs possibly giving higher scores. The utility of this randomized multiple-run approach has not been investigated.

The solution only depends on the most frequent epitopes at each position, so for the example in Table 1, it will be a consistent combination of DEF, EFG, CHJ, HJL, JLM. If they are dropped in that order (first four have frequency 2, last one has frequency 3), DECHJLM [SEQ ID NO: 810] is obtained for which the score 0+0+2+2+3=7 beats the consensus but is less than optimal. If they were dropped in the order HJL, CHJ, EFG, DEF, JLM, DEFGJLM [SEQ ID NO: 811] would be obtained with a score of 2+2+1+1+3=9, which is the optimal score for this example. These two solutions are illustrated in FIG. 3.

The Aligned Cocktail of Vaccines (M>1) Problem.

In the original mosaic solution using genetic algorithm optimization, all M of the mosaic sequences are optimized at the same time.

Sequential Solutions.

One way to extend the M=1 episensus solutions to the M>1 problem is to modify the algorithms for optimizing total coverage to optimize complementary coverage. That is: given an episensus solution q₁, find q₂ that covers as many as possible of the remaining epitopes, not covered by q₁.

Iterative Refinement of Sequential Solutions.

Given initial solutions q₁, q₂, . . . , q_(M), a new estimate for q₁ can be recomputed. This is done by starting with the original frequency values for each of the epitopes, but setting to zero those epitopes that are covered by q₂, . . . , q_(M). The optimization of this complementary coverage problem leads to a new q₁. One can loop through all of the initial solutions this way, each time optimizing the complementary coverage.

Off-by-One Scoring.

The analysis shown so far gives credit to coverage only if an epitope in a sequence s is exactly matched by an epitope in a sequence q. But, particularly for longer epitopes, e.g., k=12, an epitope in a sequence may still be effective if it is an approximate match. For instance, agreement in 11 out of 12 characters may constitute satisfactory coverage.

Results.

These algorithms were compared using a dataset of 690 sequences.

FIG. 4 illustrates what the graph looks like for such a large dataset. FIG. 4 shows a graph associated with a dataset of 690 US B-clade Gag protein sequences (SEQ ID NOs. 1-690), each aligned to 556 positions. Results are shown in Table 2. Horizontal axis is the position t, and the columns of nodes indicate the different epitopes at each position. The nodes were arranged so that the most frequent are at the bottom. The nodes and edges (indicating consistency of adjacent nodes) are shown. The full graph is shown in (a); a close-up inset of the same graph is shown in (b).

Table 2 shows a comparison of coverage scores; this is fraction of the epitopes (k=12) in the sequences S that are covered (by exact match) by the epitopes in the vaccine sequences Q={q₁, . . . ,q_(M)}.

TABLE 2 Algorithm Episensus (M = 1) M = 2 M = 3 M = 6 Consensus 0.5070 — — — Genetic Algorithm 0.5072 0.6098 0.6643 0.7308

For the global EpiGraph solutions, single best EpiGraph sequences were determined based on the M group, B clade and C clade database sequences (including upto circa 2015), as well as the complementary 2^(nd) EpiGraph sequence for a bivalent vaccine, for Gag, Pol, and Nef. A novel advantage of the EpiGraph code over Mosaic design is that it allows the deliberate exclusion of rare epitopes, a feature included in the design of the new sequences, see figure below for an example of the impact of excluding rare variants from the M group. FIG. 5A-C illustrates the final values of EpiGraph and Tailored vaccines that were used.

Example 2: Graphical Model for Optimal Epitope Coverage of Unaligned Sequences

A set S={s₁, s₂, . . . , s_(N)} of N unaligned protein sequences is taken to characterize the variability of a virus over a target population (e.g., a phylogenetic clade, a country, or global). A potential epitope is a subsequence of k amino acids (typically k=9). Each potential epitope, e, is assigned an integer frequency f(e) corresponding to the number of sequences in S in which e appears. The monovalent problem is to design a single artificial sequence q that resembles a natural protein but optimally covers the potential epitopes in the population S. Writing E(q) as the set of epitopes that appear in q, our measure of coverage is

Coverage(q)=Σ_(eεE(q)) f(e)/Σ_(eεE(s)) f(e)

The numerator is the sum of the frequencies of epitopes that appear in q, and the denominator normalizes by the sum over all epitopes that appear in any of the sequences in S. This formulation can be expressed as a directed graph. Each node in the graph corresponds to a distinct epitope e, and a directed edge connects two length-k epitopes (e_(a); e_(b)) if those epitopes overlap by k−1 characters. A path through the graph is a sequence of nodes e1, e2, with an edge from e_(i) to e_(i+1) for i=1, . . . , L−1. Such a path corresponds to a sequence of L+k−1 characters, which is the artificial antigen q.

If this directed graph has no cycles, then EpiGraph finds a path through the graph that rigorously maximizes coverage, providing the optimal solution. Furthermore, this optimization is done with computational effort that scales only linearly with the size (as measured in nodes and edges) of the graph. In practice the directed graph created from S may not be acyclic, though it is often very nearly so, especially for larger values of k. For this case, the graph was “de-cycled,” by iteratively identifying cycles and then removing low-value edges until no cycles remain.

A polyvalent “cocktail” of m>1 antigens can be created by running EpiGraph sequentially, and optimizing complementary epitope coverage. This is achieved by treating the epitopes e that were included in the first antigens as if their frequencies were zero, and then running Epigraph on these modified frequencies. If any of the epitopes in the first antigens are required to complete a path (and generate a complete protein), they will still be available, but they will be disfavored, since they no longer contribute to the coverage score. This sequential solution can be improved using iterative refinement.

Example 3: Tailored Therapeutic Vaccines

While it is not feasible to build a designer vaccine for each subject, it is feasible to sequence virus from that subject to try to get a good match from within a small reference set of vaccine options. The first thing considered was a US-based B clade trial population, focusing on the Gag protein. A Southern African based reference vaccine set, and a global vaccine set were designed, as well as an updated US-based B clade design. p24 is the most highly conserved sub-protein in Gag and can be excised from the larger Gag protein to provide a conserved region of ˜230 amino acids in length. A conserved region approach was also considered as an alternative to Gag, perhaps focusing on regions in Gag and Pol, possibly including the conserved stretch of Nef as well as any other proteins of interest.

This is a very different optimization question from trying to design a set that provides optimal population coverage for a prophylactic vaccine. In the prophylactic case, it is not known which viruses might be encountered by the vaccinee. In a therapeutic case the infecting virus sequence can be obtained and matched.

Optimization was done considering two things: first, to maximize the matches from a subject's infecting viruses and second, to minimize the mismatches so that the vaccine response is as targeted as possible on the relevant epitopes.

The phylogeny within HIV major clades tends to have little clear structure.

Rather it is a “starburst” with very long external branches, and very short poorly defined internal branches near the base. Part of this structure is likely due to intra-subtype recombination. While that is hard to quantify, recombination is certainly occurring relatively frequently, and by analogy with what is seen in terms of inter-subtype recombination, it is likely to be extensive. Given the structure of the tree, simply using clustering on a phylogenic tree to define the reference set of possible vaccines will not be as effective because within-clade associations are of limited meaning from an “epitope perspective”. Instead, sequence relationships should be considered by the relevant measure, and the reference set should be selected based on potential epitope similarities between natural strains and putative vaccine designs.

12-mers were optimized considering class II epitopes, but the code can use any length k-mers as a reference point, where k is the putative potential epitope length. 9-mers were also used. In past work with the mosaics, the optimal solution for 9-mers has been very nearly optimal for other nearby lengths (8, 10, 11, 12), and that is expected to carry over with the new algorithms described here. 9-mers have been used for certain Tailored vaccine sets disclosed herein.

Optimality was defined in terms of k-mer coverage. This is defined by replacing each sequence with a “bag of epitopes,” i.e., an unordered list of all the k-mers that appear in the sequence. A bag of epitopes can be defined for a set of sequences by making a list of all k-mers that appear in any of the sequences in the set. The coverage of a given sequence S by a set of sequences Q={q₁, q₂, . . . , q_(N)} is given by the fraction of epitopes in S's bag that are also in the collective bag of the Q sequences, where Q might be a polyvalent combination in a vaccine cocktail. It is this coverage that was optimized. Another quantity of interest was the fraction of epitopes in the Q-bag that are not in the S-bag. Although it is not (currently) used directly in the optimization, a smaller fraction of these extraneous epitopes is preferred, and these numbers are calculated for comparisons and experimental design.

Six (6) ways of finding “central sequences” were evaluated when performing clustering for a Tailored vaccine incorporated into the Tailored vaccine analyses code (see below). An EpiGraph solution was deemed best, and was used for the final code. Several clustering strategies were also tried.

Here are the strategies tested to define amino acid based central sequences of clusters:

1) Consensus: The consensus was a common standard, obtained by concatenating the most common amino acid in an alignment.

Potential Epitope (k-Mer) Based:

2) Episensus: This approach solves for a single central sequence within a population or within a cluster. Two algorithms were tested for finding the episensus. The first is the drop-in-place algorithm. This starts with the consensus as a “substrate”. Excluding very rare k-mers, (those that are only found 1 time in the population), start with rare low frequency variants and replace the consensus k-mers with the rare variant, then keep replacing with variants, going up through the list of all k-mers based on their frequency, replacing and overwriting with more and more frequent variants until the most frequent variants across k-mers are left “standing”; overlapping k-mers with higher frequency will naturally tend to override peptides that overlap with lower frequencies. The second algorithm is EpiGraph, was described in Example 1. Epigraph sequences are more quickly calculated than GA mosaics, and so were readily incorporated into a clustering algorithm required to design a Tailored vaccine. In an experiment with a set of 690 aligned sequences of 556 amino acids that comprise the Gag protein (SEQ ID NOs: 1-690), the EpiGraph solution was very close to a consensus, and was also very close to the single best mosaic, with only one amino acid difference from the other two centroids.

3) Sequential: This approach solves for a set of N vaccine option sequences. Here, the episensus is first defined for the population, and this is q₁. Next, all k-mers that are already covered by q₁ are excluded, and the approach solves for the second sequence in the series by the same drop-in-place process, to yield q₂. Then all k-mers already covered by q₁ and q₂ are excluded, and the approach solves for q₃ and so on until it has been solved for a set of N sequences, each including ever rarer versions of the potential epitopes.

4) Iterative: This is an iterative version of the sequential algorithm. It also produces a set of N vaccine option sequences, usually with better coverage than the sequential approach, but without the preferential ordering that the sequential algorithm produces. Starting with a sequential solution q₁, q₂, . . . , q_(N), all the epitopes that are in the data sequence set are identified, and then the epitopes that are covered by q₂, . . . , q_(N) are excluded. By solving for the episensus using the remaining (non-excluded) epitopes, a new value is obtained for q₁. The next step does the same thing with q₂, excluding epitopes in q₁, q₃, . . . , q_(N). And so on for q₃ through q_(N).

5) Mosaic (GA mosaic): defined using the original genetic algorithm (GA). Mosaic refers to the genetic algorithm termed Mosaic and/or antigen sequences produced by the GA. Here, a set of mosaics of size N are solved for all at once, or the best single mosaic can be solved for, fixed, and solved for a complementary set of 5 to make a total of 6 vaccine option sequences. This strategy was employed to enable direct comparisons with clustering strategies described below.

6) Best natural: This approach solves for a set of N vaccine option sequences. The natural strain in a set is identified that is most “epitope centric”, i.e. it has the most common k-mers. To find it, for every k-mer in a given natural strain, a frequency can be assigned to that specific form based on its frequency in the population, and then sum the frequencies as a measure of how well the natural strain covers the population. The natural strain with the highest score is the best single strain, Nat.1. Then, the k-mers that are covered by Nat.1 can be eliminated from the scoring scheme, and the best complement to Nat.2 can be picked by finding the best natural strain for epitope coverage excluding those epitopes already covered by Nat.1. This is done iteratively, so k natural strains are picked, where k is the number of vaccine options wanted to be considered, and they are ordered so Nat.1 is the best single strain, Nat.2 the best complement to Nat.1, Nat.3 the best complement to (Nat.1+Nat.2).

Vaccine options have been explored that are designed according to the following general strategies, comparing new ideas to specifically address therapeutic vaccine population sequences that have been designed in the past to optimize for population coverage.

If all subjects were to get the same vaccine (this might work better with conserved regions only), sequencing and tailoring are not done—these are universal designs, not optimized for each individual:

Consensus: Find a single universal sequence that best covers the population. Candidates for this 1-universal sequence included: a population consensus, the best single GA mosaic, the episensus, and the most “epi-centric” natural strain.

EpiGraph: Find either 2 or 3 population-based sequences and give them to everyone in the population. This population-based strategy was compared using the GA mosaic, the best natural strains, and the sequential and iterative mosaic solution. Since EpiGraphs are now available, and an improvement over Mosaics as we can also exclude rare epitopes, we use them.

In contrast, for a Tailored vaccine, each individual would get only the vaccine sequences that best matched his or her infection: Manufacture, for example, 6 vaccine antigen sequences, and pick the best one, or the best combination of 2 or 3 from among those 6 for delivery to the patient; i.e., choose those that provide the best coverage of a patient's infecting strain, with the fewest mismatched epitopes. Several strategies for this scenario were explored.

a. Cluster sequences with a k-means-like strategy to create 6 clusters, 1500 iterations were done (each of these iterations was a trial split-and-merge step followed by a few regular iterations) for the final sets, defined centroid sequences from these 6 clusters for vaccine sets. The distance between two sequences was defined as one minus the coverage of one sequence's epitopes by the other sequence. Initially, 6 randomly selected natural strains were used to seed the clusters. This gave a very highly related set of 6 centroid sequences. It was determined that if more natural diversity was represented, this would create a better set of reagents to create tailored vaccines. Next, the 6 clusters were seeded with most complementary 6 natural (Nat6) strains as these are very distinctive, and then reassigned the center based on the clusters as the episensus, and iteratively re-clustered, and re-centered. This strategy was compared to starting with the 6 best natural strains and using a consensus as the cluster centroid instead of the episensus, and the episensus gave slightly better coverage. Enforcing a minimum cluster size also gave slightly better coverage (1, 5 and 20 as minimum cluster sizes were tried), so a minimum cluster size was incorporated as a constraint. A minimum size of 20 was better than 1 or 5. To implement a minimum size, if in a given cycle the number of sequences in a cluster size falls below the minimum size, the members of this “too-small cluster” are each reassigned to best centroid from the other five clusters. To make a new cluster to replace the one that was lost, the cluster that has the greatest average distances to its centroid was split by taking two random natural sequences from within the cluster as centroids and reforming two clusters about them, recalculating the centroids, and going on to the next step with these new six clusters. The centroids of these clusters were very close to the center of the tree. It was determined from the sequences that this is due to replication of the consensus repeatedly within the clusters dominating the signal.

b. Episensus+(5 cluster centers). Here, the central sequence was first defined for the whole population using the EpiGraph algorithm (the population episensus), fixed for inclusion as a vaccine reagent. Any epitopes that match the population episensus were excluded from clustering considerations. Sequences are clustered as before, with a minimum size but this time 5 clusters based on all potential epitopes except those found in the episensus, so the clusters complement the population episensus, were targeted. This was determined to be the best solution for Gag. By including the population episensus in each subject's tailored vaccine, even if a given most common k-mer is not evident in their sampled HIV sequences, it might be lurking or a common form for reversion, given HIV's frequent amino acid toggling between common forms. The second complementary sequence from one of the 5 clusters would then add to the variant cross-reactive potential between the vaccine and the infecting strain.

c. Fix the best EpiGraph, add 5 mosaic complements to get a set of six (the 5 added are not ordered), or fix the best natural, add 5 natural in series.

After extensive comparisons and refinements, we favored using the EpiGraph algorithm to define the center of clusters for Tailored vaccines. A set of 6 Gag protein antigens for manufacture in a Tailored design, targeting either a global M group vaccine, a contemporary B clade vaccine, and a contemporary C clade vaccine for Southern Africa are provided. A comparison of coverage of natural contemporary B clade sequences using 2 natural B clade sequences, M group EpiGraphs, B clade EpiGraphs, or B clade tailored is shown in the FIG. 6A-B; B clade Tailored designs provide the best epitope coverage.

The various scenarios can be summarized with a few numbers as seen in Table 3, which are denoted M, C, T, A, and P. These are described below in terms of the number of “pills” (ie, vaccine antigens) for each category:

M=Manufactured, total number of pills created, from which some subset is chosen for each individual. (Typically, we imagine M=6 or fewer.)

C=Common, those pills that everybody gets, possibly in addition to some tailored pills.

T/A=Tailored/Alternatives, T is the number of tailored pills (out of A alternatives) that are given to each individual, possibly in combination with some common pills.

P=Per-subject total (T+C), the number of pills given to each individual.

TABLE 3 COVER EXTRA DELTA Centroid P C T/A M >good >bad >good Common: everyone gets the same population-based vaccine: Consensus 1 1 0/0 1 0.5070 0.4913  0.0157 1-GA Mosaic 1 1 0/0 1 0.5072 0.4911  0.0161 Episensus 1 1 0/0 1 0.5065 0.4918  0.0147 Natural 1 1 0/0 1 0.4793 0.5191 −0.0398 2-GA Mosaic 2 2 0/0 2 0.5960 0.6692 −0.0732 2-iterMosaic 2 2 0/0 2 0.5965 0.6346 −0.0381 2-Natural 2 2 0/0 2 0.5473 0.6584 −0.1110 3-GA Mosaic 3 3 0/0 3 0.6346 0.7424 −0.1078 3-iterMosaic 3 3 0/0 3 0.6534 0.7190 −0.0656 3-seqMosaic 3 3 0/0 3 0.6429 0.7119 −0.0690 3-Natural 3 3 0/0 3 0.5941 0.7308 −0.1367 6-interMosaic 6 6 0/0 6 0.7205 0.8324 −0.1119 1 + 5-GA Mosaic 6 6 0/0 6 0.7048 0.8241 −0.1193 6-Natural 6 6 0/0 6 0.6628 0.8104 −0.1476

In Table 3, the single underlined numbers indicate that on average >60% of the 12-mers in natural sequences are covered by the vaccine (GOOD), while the double underlined numbers indicate that over 70% of the 12-mers on average are not present in the natural stains.

Tailored—Choose the Best 1 of 6 for Each of the 690 Population Sequences:

Not much better (0.54 vs 0.51) than just making one for the whole population, but C6-epi is the best of class if 6 vaccines were going to be made, and give one of the six to a subject based on their sequence.

C6-epi 1 0 1/6 6 0.5366 0.4616 0.0750 1 + 5-Epi-C5 1 0 1/6 6 0.5079 0.4904 0.0175 1 + 5-GA Mosaic 1 0 1/6 6 0.5083 0.4900 0.0183 1 + 5-iterMosaic 1 0 1/6 6 0.5074 0.4909 0.0165 6-iterMosaic 1 0 1/6 6 0.3979 0.6008 −0.2029  6-Natural 1 0 1/6 6 0.4863 0.5122 −0.0259  C6-NaturalSeed 1 0 1/6 6 0.5291 0.4692 0.0599 Tailored Best Pair from a Group of Six

C6-epi 2 0 2/6 6 0.5785 0.5313  0.0472 1 + 5-Epi-C5 2 0 2/6 6 0.6255 0.6310   −0.0055 ** 6-interMosaic 2 0 2/6 6 0.5552 0.6859 −0.1307 6-seqMosaic 2 0 2/6 6 0.6075 0.6331 −0.0256 1 + 5-GA Mosaic 2 0 2/6 6 0.5932 0.6401 −0.0469 6-Natural 2 0 2/6 6 0.5759 0.6431 −0.0672 Hybrid tailored pair: fix the population center, add 1 of 5 centroids to

best complement a fixed sequence to cover each of the test sequences—

1 + 5-Epi-C5 2 1 1/5 6 0.6255 0.6310   −0.0055 ** 1 + 5-GA Mosaic 2 1 1/5 6 0.5922 0.6394 −0.0472 1 + 5-Natural 2 1 1/5 6 0.5724 0.6395 −0.0671 6-seqMosaic 2 1 1/5 6 0.6071 0.6330 −0.0259 6-iterMosaic 2 1 1/5 6 0.5280 0.7039 −0.1759 Hybrid best three—fix the population center, and 2 of 5 centroids to best complement a fixed sequence so cover the test sequence.

1 + 5-Epi-C5 3 1 2/5 6 0.6590 0.6868 −0.0278 HBP-6-iterMosaic 3 1 2/5 6 0.6586 0.7079 −0.0493 HBP-1 + 5-GA Mosaic 3 1 2/5 6 0.6447 0.7155 −0.0708 Common: Everyone gets the same population-based vaccine, either 1 or 2 vaccine antigens are delivered:

Common Natural 1 1 0.4793 0.5191 Common Mosaic 1 1 0.5072 0.4911 Common 2-Natural 2 2 0.5473 0.6584 Common 2-GA Mosaic 2 2 0.5960 0.6692 Common 2-iterMosaic 2 2 0.5965 0.6346 Tailored C6-epi 2 6 0.5785 0.5313 Tailored 1 + 5EpiC 2 6 0.6341 0.6361 Tailored 1 + 5EpiC5 3 6 0.6590 0.6868 Common 3-GA Mosaic 3 3 0.6346 0.7424

Exact Match 1+5-Epi-05:

Optimized for 9 Optimized for 12 COVER EXTRA COVER EXTRA Evaluated for 9 0.7098 0.5744 0.7066 0.5535 Evaluated for 12 0.6301 0.6525 0.6341 0.6361

And Off-by-One Considered a Match 1+5-Epi-05:

Optimized for 9 Optimized for 12 COVER EXTRA COVER EXTRA Evaluated for 9 0.9207 0.1783 0.9183 0.1723 Evaluated for 12 0.8722 0.2590 0.8708 0.2474 ** these are likely the best solution. A population episensus was made, the 12-mers found in the episensus for clustering were excluded. When the population episensus is fixed and the best episensus of the other 5 complementary clusters is picked to pair with it, the exact same answer is obtained as when the best pair among those 6 variants was picked. This means the population episensus was always one of the best pair.

Off by One:

For the estimates above, only perfect matches were considered, for an epitope to match between a vaccine cocktail and a natural strain, a perfect 12/12 match was required. Mismatches are often well tolerated, particularly for class II epitopes, if a match requires 11/12 agreement, a mismatch is 10/12 or less, things look more optimistic. The truth is probably somewhere in between, and 10/12 may be acceptable is some cases as well. Here the likely best option is compared to a comparable best natural strain option, with perfect matches:

Perfect Match (Extracted from the Table Above):

1 + 5-Epi-C5 2 1 1/5 6 0.6255 0.6310 −0.0055 ** 1 + 5-Natural 2 1 1/5 6 0.5724 0.6395 −0.0671  

And Off-by-One Considered a Match:

1 + 5-Epi-C5 2 1 1/5 6 0.8700 0.2524 0.6176 1 + 5-Natural 2 1 1/5 6 0.8412 0.3112 0.5300

Three pills, one general (same for everybody). And two tailored pills, the best pair of the remaining five.

Example 4: Design and Optimization of a Clade B HIV-1 “Tailored” Antigen Cocktail

HIV diversity at the population level begins with rapid evolution within each infected host. Much of the within host diversity is a direct consequence of immune escape (Bar, K. J. 2012. PLoS Pathog 8:e1002721., Liu, M. K. 2013. J. Clin. Invest. 123:380-93, and escape variants arise soon after infection (Fischer, W. 2010. PLoS One 5:e12303). The goal of prophylactic vaccines is to rapidly eliminate the infecting strain or prevent it from infecting in the first place. Since it is not known which strain will be transmitted, prophylactic vaccines have to elicit immune responses that would be active against any variant that might be encountered. In contrast, an immunotherapy can be directed against a known viral population present within a given host. Prophylactic and therapeutic HIV vaccines thus have to be designed with these distinct requirements in mind. As shown in FIG. 7, mosaic solutions approach the maximum possible epitope coverage for a given HIV population. The figure also reveals differences in the conservation of HIV proteins, with large segments of Gag, Nef and Pol being highly conserved whereas most of Env as well as most small, auxiliary proteins (data not shown) are highly variable. While mosaics offer a near optimal solution for epitope coverage in a population for a prophylactic vaccine, they do not exploit the added information of knowing the sequence of the infecting strain targeted in a therapeutic setting.

The implementation of a tailored vaccine design strategy requires manufacturing a manageable number of vaccine reagents that maximally capture the epitope diversity of the target population, sequencing HIV from the infected vaccine recipient, and then selecting from among the vaccine reagent pool the subset of antigen inserts that best matches the subject. The tailored vaccine strategy is thus conceptually distinct from mosaic vaccine design.

98% of approximately 150,000 HIV sequences that have been sampled in the U.S. are clade B. The US-sampled B-clade Gag sequences currently available from the Los Alamos HIV database were used. There were 690 intact Gag sequences in this set (SEQ ID NOs. 1-690), each derived from a different infected subject, representing a cross-section of the diversity in US HIV strains. To maximize T cell epitope coverage in a hypothetical vaccine while fixing the number of different Gag sequences that need to be contained in a vaccine cocktail, a novel computational strategy was devised that couples the use of an epitope-based consensus (or “episensus”) sequence algorithm with k-means-like clustering. Unlike a simple consensus sequence, which takes the most common amino acid in each position of the alignment, the episensus seeks the most common epitope (e.g., 12-mer) starting in each position of the alignment. However, one cannot simply take the most common epitope at each position, because the epitopes overlap, and that can lead to conflicts in nearby amino acids. The goal is to deal with those conflicts in a way that leads to maximal epitope coverage of a population by a vaccine.

To define a small set of antigens for manufacture that represent the population epitope diversity, a k-means like approach was used to partition the 690 Gag sequences into distinct clusters based on epitope similarity, and separate episensus sequences were generated for each cluster to serve as the central sequence of the cluster. Coverage of individual variants levels off after 5-6 clusters, and 6 vaccine vectors with 6 HIV antigen variants is feasible to manufacture, so a set of 6 antigens was initially targeted. The algorithm starts with 6 randomly selected natural strains as initial centroid sequences, and assigns each of the remaining sequences to whichever of the 6 is closest. Within each of the 6 clusters, a new centroid sequence is computed, based on the episensus of the sequences in that cluster. All of the sequences are then re-assigned according to which of the new centroids is closest. This process converges to a k-means-like optimization for the design of the 6 vaccine antigens, which are taken to be the episensus centroids of the individual clusters, and these antigens could be used as a manufactured set, from which to select those that provide the best-match for a given vaccine.

A hybrid approach was found to substantially improve epitope coverage, while minimizing increases in potential vaccine-specific responses. In the hybrid strategy, the central sequence was computed for the whole population (the population episensus), and subsequent clustering was based exclusively on epitopes that were not found in the central sequence. In this way, the cluster episensus sequences complement the population episensus. Thus, the 6-sequence reagent pool consists of the one population episensus and five complementary-cluster centroid sequences, and subjects would be given a two-antigen tailored vaccine, the population episensus paired with the best complementary sequence. This strategy yielded more diverse sequences in the reference set, and improved potential coverage of natural Gag sequences in the test population as seen in Table 4.

TABLE 4 Average per-strain coverage of potential epitopes in US clade B Gag by different vaccines. % non- % matched to matched to Improvement Vaccine Delivered Manufactured natural Gag natural Gag factor 1 Natural 1 1 47.9 51.9 1 Mosaic 1 1 50.7 49.1 1.06 1 Episensus 1 1 50.7 49.1 1.06 2 Natural 2 2 54.7 65.8 1.14 2 Mosaic 2 2 59.6 66.9 1.24 2 Tailored 2 6 63.4 63.1 1.32 3 Natural 3 3 59.7 73.1 1.35 3 Mosaic 3 3 63.4 74.3 1.32 3 Tailored 3 6 65.9 68.7 1.37 6 Natural 6 6 66.3 81.0 1.38 6 Mosaic 6 6 72.1 83.2 1.51

A tailored vaccine, 2- or 3-antigen design, was made for each of the 690 variants, selected from the population episensus and the 5 cluster derived complementary sequences. This was compared to the potential epitope coverage of population-based vaccine strategies where every vaccinee would be given the same vaccine, either mosaic or best-natural combinations. “Delivered” indicates the number of antigens that would be included in a vaccine cocktail. “Manufactured” indicates the size of the reference pool of vaccine antigens that would need to be synthesized to choose among for the tailored approach to be used. The “% matched” indicates the average number of potential epitopes perfectly matched between each of the natural Gag variants and the vaccine; the higher this value, the more likely vaccine responses will be cross-reactive with natural variants. The “% non-matched” represents the fraction of potential epitopes in the vaccine that are not found in a given natural Gag sequence, calculated for each of the 690 sequences separately then averaged. The higher this value, the greater the potential the vaccine to elicit vaccine-specific responses that may detract from cross-reactive responses. The improvement factor indicates the increase in coverage using the proposed new vaccine design options, over using a single best natural strain.

The tailored vaccine solutions optimized on 12-mers are nearly optimal for 9-mers, and vice-versa, so clusters based on 12-mers should work well for both class I and class II epitope presentation as seen in Table 5.

TABLE 5 Allowing a single amino acid mismatch in the 9mer or 12mer evaluation to be considered as a positive match when calculating the average coverage of a 2-protein tailored design. Optimized for 9mer Optimized for 12-mer % Non- % Non- % Matched matched % Matched matched Evaluated for 9-mer 92.07 17.83 91.83 17.23 Evaluated for 12- 87.22 25.90 87.08 24.74 mer

Finally, the tailored vaccine approach theoretically does very well if 1 out of 12 mis-matches are tolerated in potential epitopes, rather than requiring identity (Table 5); given this more lenient, and perhaps biologically more realistic, measure, approximately 90% of vaccine responses to tailored vaccines may be cross-reactive with epitopes in matched natural Gags. The tailored vaccine approach was superior to population mosaics, consensus sequences, and the best natural strains in terms of both maximizing epitope coverage of Gag sequences, and minimizing potentially deleterious vaccine-specific epitopes (FIG. 8). The code can also be applied to tailoring vaccines using multiple sequences rather than use one representative each from infected individuals, and applying the tailored vaccine design strategy to different populations (the C clade epidemic in Southern Africa, the 2-clade regional epidemic in Thailand, and global M group set) are explored.

Tailored vaccine antigens can provide better coverage (compared to population-based antigens) of natural sequences when the infecting strain is known.

Example 5: Dual Expression Vectors

Using the population episensus antigens and/or the tailored antigens described herein, dual expression vectors are generated, with each expressing a complete Gag antigen and a second HIV antigen. The second HIV antigen can be, for example, a fusion protein of reverse transcriptase (RT) and the central part of Nef. Integrase is not included, as it is a rather poor stimulator of T cell responses. Using CMV vectors (e.g., RhCMV or HCMV vectors) as dual expression vectors, it is possible to simultaneously induce T cells to two different SIV or HIV antigens.

For example, panels of up to six HCMV vectors containing tailored Gag sequences based on the EpiGraph algorithm developed are generated (one vector expressing a Gag population episensus antigen and five vectors that each express a complementary cluster-based Gag antigen), and panels of antigens covering RT and the central region of Nef are also designed. One vector expressing a Gag population episensus antigen plus two vectors each expressing a different Gag antigen can be selected from among the five complementary cluster-based antigens are provided. These vectors can also contain one of two complementary HIV-1 EpiGraph RT/nef sequences. When tailoring is not predicted to improve coverage due to the high conservation of these sequences, the 2-mosaic solution are retained and used for the vector. For example, one RT/nef mosaic is included in the population episensus vector and the other is used in the tailored vectors. A panel of HCMV-based vaccine vectors that can enter vaccine production is generated by sequencing the resulting vectors and characterizing them for antigen expression and growth in vitro.

Synthetic codon-optimized DNA inserts are generated corresponding to Gag, RT and Nef mosaic and the tailored antigens designed in Example 3.

Example 6: Transient Expression of Viral Antigens Developed Using the EpiGraph Approach

Antigens designed to maximize the epitope frequency using the EpiGraph algorithm resemble natural sequences but no longer code for native proteins. While the theoretical guidelines for expression of these artificial sequences are adhered to in the construction of these sequences, proteins encoded by them may exhibit unanticipated expression profiles or fail to express a stable full length protein.

To evaluate the expression profile of these sequences in the context of mammalian cells EpiGraph sequences were synthesized and cloned for transient transfection. DNA encoding these constructs was synthesized (Genscript, Piscataway, N.J.) to contain compatible cloning sites for plasmid vectors (pcDNA3.1 and pOri). All inserts were codon optimized for the respective host (rhesus, SIV or human, HIV). Each construct was also modified to eliminate residual enzyme activity of the native sequence as described in Kulkarni et al. Vaccine (2011). Positions deleted were based on the amino acid sequence relative to Clade B EpiGraph-1. Amino acids deleted include; “DTG” associated with protease activity (positions 81-83), “YMDD” associated with reverse transcriptase activity (positions 338-341), “E” associated with RNaseH activity (position 633), “D” associated with Integrase activity (position 779), “D” associated with Integrase activity (position 831), and “E” associated with Integrase activity (position 867). Synthetic DNA was rehydrated in water and digested with restriction endonucleases (5′ NheI, 3′ BamHI) followed by heat inactivation. The plasmid vector was linearized with compatible endonucleases and treated with calf intestinal phosphatase to prevent recircularization of empty vector. Vector and insert fragments were resolved by agarose gel electrophoresis to confirm digest fragment sizes and cleaned for ligation by PCR purification kit (Thermo Scientific). Inserts were ligated to linearized vector at approximately 3:1 insert to vector ratio for 15 minutes at room temperature using a rapid ligation kit (Roche, Indianapolis, Ind.), transformed into chemically competent E. coli (DH5-alpha), and plated on antibiotic selection plates. DNA from resulting colonies was screened by restriction digestion for inserts.

Clones containing each of the correct inserts in the appropriate orientation relative to vector promoter and poly(A) sequences were grown in liquid culture for plasmid DNA purification. Actively growing sub-confluent Hela cells in 12 well tissue culture plates received 500 ul of fresh media (DMEM 10% FBS) while liposomes were prepared. To generate liposomes containing plasmid DNA, 250 ul of serum free media was mixed with 500 ng of plasmid DNA, and 250 ul of serum free media was mixed with 2 ul of lipid (Lipofectamine 2000, Invitrogen). After 5 minutes incubation at room temperature these solutions were combined, mixed, and incubated for 20 minutes. The DNA containing liposomes (500 ul) formed during this process were added dropwise to the culture and allowed to incubate 12-16 hours after which time the transfection mixture was replaced with fresh media. After an additional day of incubation cultures were harvested by scraping and centrifugation. Supernatants were removed by aspiration and cell pellets lysed by resuspension in 100 ul gel loading dye containing 5% SDS and 10% 2-mercaptoethanol and centrifugation through QiaShred column (Qiagen, Valencia, Calif.).

Expression of EpiGraph proteins was demonstrated by SDS poly-acrylamide gel electrophoresis (SDS-page) and western blotting developed with antibodies to the V5 or hemagglutinin epitope tag engineered into each construct. Briefly, 10% polyacrylamide gels were prepared and loaded with 10 ul (10% of each sample) and electrophoresed at 110-120 volts for 90 minutes. The resolved proteins were transferred to PVDF membranes by semi-dry blotting at 20 volts for 45-50 minutes. Non-specific binding was blocked with a solution of 10% nonfat dry milk in phosphate buffered saline with 0.1% tween-20 (PBS-T) for 60 minutes. HA (Sigma) or V5 (Santa Cruz) antibodies were diluted in 5% milk solution and incubated with membranes for 1 hour followed by 3 washes with PBS-T prior to addition of 1:2000 dilution of horseradish peroxidase conjugated goat anti-mouse (Santa Cruz) secondary antibody for 1 hour. Subsequently blots are washed three times in PBS-T and developed with enzyme linked chemi-luminescence (ECL kit (Thermo-Pierce) and visualized with X-ray film.

All the tested constructs demonstrated robust transient expression for proteins of the predicted molecular weight and confirmed their utility for testing in the CMV vector backbone. (for example, see FIGS. 9A-C).

Example 7: Engineering of EpiGraph Designed Antigens into CMV Vector BAC Constructs and Expression from Reconstituted Virus

EpiGraph antigens were designed to maximize the coverage of T-cell epitopes representative of the spectrum of viral sequences and clades of HIV from which they were generated. To utilize these antigens most effectively they have been engineered into CMV vectors which have demonstrated three times the CD8⁺ T cell spectrum of competing platforms. Broad antigen presentation and lifelong expression profiles of CMV vectors have demonstrated the capacity to protect and cure rhesus monkeys infected with SIV. The EpiGraph antigen design algorithm in combination with CMV vectors may provide even greater coverage of HIV within and across clades when applied to broadly prophylactic vaccines or tailored focused vaccines.

EpiGraph sequences which were demonstrated expression in transient transfection systems were sub-cloned into the recombination plasmid (pOri) and transferred to CMV backbones using BAC recombineering. (Messerle et al. Proc Natl Acad Sci USA. 1997 Dec. 23; 94(26):14759-63; and Borst et al. J Virol. 1999 October; 73(10):8320-9).

BAC recombineering facilitates the manipulation of large DNA sequences utilizing temperature and metabolite regulated recombination enzymes in the context of E. coli strain EL250 containing a parental BAC. Recombination is a sequential two-step process consisting of insertion of the antigen sequence with an antibiotic resistance gene (kanamycin) into the target region followed by removal of the kanamycin cassette. The insertion fragments are amplified by PCR from template DNA containing the antigen of interest plus kanamycin using primers with long (50+bp) homology arms.

To prepare the bacterial cells for the insertion step, five ml cultures were grown overnight at 30° C. in Luria Broth (LB) with chloramphenicol, and diluted up to 50 ml the following morning. Bacteria were grown for approximately 3-4 additional hours at 30° C. (to an OD=0.6), and then heat shocked by shaking at 42° C. for 15 minutes to induce the recombination enzymes. Following this induction, bacteria were pelleted (3000 rpm, 10 minutes, 4° C.) and then washed three times in ice-cold water. The E. coli cells were rendered electro-competent to receive the PCR product and recombination competent for insertion of the sequence into the target region of the BAC. Purified insert (500 ng) was combined with 100 ul competent E. coli on ice, moved to a 0.2 cm cuvette (Fisher), and electroporated using the Bio-rad MicroPulser apparatus. Following electroporation, the bacteria were diluted by addition of 900 ul LB culture media and allowed to recover at 30° C. for 2 hours prior to plating on chloramphenicol/kanamycin plates. Plates were incubated at 30° C. for two days and colonies were screened by restriction digest and PCR for recombination events.

BAC constructs positive for recombination proceeded to the second step where the kanamycin cassette was excised by arabinose induction of the Flip recombinase mediated by flanking FRT sites. Five ml cultures were grown overnight in LB+chloramphenicol and diluted 1:10 the following morning. After three hours of growth the bacteria were treated with L-arabinose (Arcos, 0.1% final concentration) and induced for 1.5 hours at 30° C. Following induction the bacteria were streaked on chloramphenicol plates and incubated for two days at 30° C. Colonies were then replica plated on chloramphenicol/kanamycin and chloramphenicol plates to screen for clones that had lost kanamycin resistance. These clones were further screened by restriction digest and PCR to confirm the construct.

Viral Reconstitution:

To regenerate virus, the BAC DNA was transferred into mammalian host cells permissive for viral growth. BAC DNA purified from 10 ml of an overnight culture was electroporated into approximately ⅕ of a confluent flask of telomerized fibroblasts (˜200,000 cells). In brief, cells were pelleted (1,500 rpm, 5 minutes) and resuspended in 700 ul Opti-Mem. This cell mixture was then added to 50 ul of BAC DNA and mixed gently before transfer to a 4 mm cuvette. Electroporation was done using the Bio-rad GenePulser II at 0.25 kV and 0.95 uF. Following electroporation, cells were plated into 100 mm dishes containing DMEM+10% FBS and media was changed the next day to remove cell debris. Cells were observed daily for the formation of plaques and harvested at full CPE. The remaining attached cells were harvested by cell scraper and pelleted by centrifugation (1,500 rpm, 5 minutes), and the supernatant containing reconstituted virus vector was retained for passage of the recombinant virus. Cell pellets were lysed by resuspension in 100 ul gel loading dye containing 5% SDS and 10% 2-mercaptoethanol and centrifugation through QiaShred column (Qiagen, Valencia, Calif.).

Viral EpiGraph Expression:

Expression of EpiGraph proteins were tested by SDS poly-acrylamide gel electrophoresis (SDS-page) and western blotting developed with antibodies to the V5 or hemagglutinin epitope tag engineered into each construct. Briefly, 10% polyacrylamide gels were prepared and loaded with 10 ul (10% of each sample) and electrophoresed at 110-120 volts for 90 minutes. The resolved proteins were transferred to PVDF membranes by semi-dry blotting at 20 volts for 45-50 minutes. Non-specific binding was blocked with a solution of 10% nonfat dry milk in phosphate buffered saline with 0.1% tween-20 (PBS-T) for 60 minutes. HA (Sigma) or V5 (Santa Cruz) antibodies were diluted in 5% milk solution and incubated with membranes for 1 hour followed by 3 washes with PBS-T prior to addition of 1:2000 dilution of horseradish peroxidase conjugated goat anti-mouse (Santa Cruz) secondary antibody for 1 hour. Subsequently blots were washed three times in PBS-T and developed with enzyme linked chemi-luminescence (ECL kit (Thermo-Pierce) and visualized with X-ray film.

All the tested constructs demonstrated robust stable expression for proteins of the predicted molecular weight, thus confirming their utility for immunogenicity testing in the rhesus CMV vaccine model. (for example, see FIG. 10A-B).

Example 8 Population Epigraph Vaccines

The Epigraph algorithm were used to create a set of vaccine antigens using CMV vectors initially, however, other vaccine delivery systems can be utilized.

M group (global) was considered, as well as B and C clade (geographically limited use to regions where these particular clades are endemic). Gag, Pol and Nef Epigraph vaccine antigens were generated −. B and M group are expressed.

Basic Epigraph Design Attributes:

Epigraphs use a graph theory/dynamical programming approach to design antigens that maximize potential T-cell epitope (PTE) coverage. Under certain conditions they are mathematically optimal, and they are very computationally efficient. Epigraphs have an additional tangible benefit relative to Mosaic antigens, in that the benefit of excluding ever-more rare epitopes in the constructs can be balanced by tolerating minimal PTE coverage costs. These Epigraphs were designed with that in mind, allowing a slight coverage cost (0.005) to ensure that even the rarest epitopes represented in the Epigraph antigens were observed in many of the population sequences (the precise number depends on the input data set).

The input data sets for these Epigraphs were obtained from the HIV database sequence alignment set for each of the proteins, Gag, Pol and Nef, including one sequence per person, circa September 2014. Incomplete sequences were excluded. This left the following numbers of sequences for each protein set, Nseqs is the number of sequences in the input alignment:

Nseqs Clade Protein 1729 B Gag 1780 B Nef 1072 B Pol 940 C Gag 749 C Nef 414 C Pol 4596 M Gag 4040 M Nef 2780 M Pol

Paired Epigraph antigen sets for a bivalent vaccine were sequentially solved using the Epigraph algorithm for unaligned sequences. The sequential solution was used, which allows the use of first Epigraph as a monovalent vaccine in isolation. This means that they are designed so that the best single Epigraph antigen, an “episensus”, is solved first to provide the optimal PTE coverage of a population, and then it is fixed for inclusion in the bivalent design. The complement is then solved to give best population PTE coverage by a bivalent pair of antigens that contain the first Epigraph, the episensus. The coverage costs were minimal relative to a simultaneous antigen solution.

An analysis was then performed to determine the coverage cost of excluding rare variants. The data is summarized in the following table. f_(o) is rare epitope threshold. Sequences are produced after discarding all PTEs that appear in f_(o) or fewer sequences. Put another way, every PTE that is in the vaccine has appeared in more than f_(o) sequences. These values off, were made as large as possible while achieving a coverage that was within 0.005 of the maximum coverage achieved when f_(o)=0. Nseqs is the number of sequences in the input alignment.

Protein Clade Nseq f_(o) Gag B 1729 41 Gag C 940 21 Gag M 4596 146 Nef B 1780 50 Nef C 749 14 Nef M 4040 100 Pol B 1072 34 Pol C 414 11 Pol M 2780 67

The basic Epigraph antigens were designed as full proteins, and these—were—expressed in CMV vectors and tested as either Gag/Nef fusion proteins, or Pol with deletions made for safety, or as the most conserved regions of Gag and Nef fused, or the most conserved regions of Pol fused. Lists of each of the full protein form for expression in the CMV vector are included below with examples for the M group and the B clade.

Conserved regions for vaccine antigens are excised from the full length Epigraph proteins.

The conserved regions within Gag, Pol and Nef were defined based on the potential for PTE coverage by a bivalent (i.e., 2 antigen) vaccine. That is, they were based on the potential for two optimized antigens to provide PTE coverage of the B clade. Sequences for the conserved regions are shown in the listing below. Boundaries were selected to capture the most conserved half of each the three proteins (Gag, Pol and Nef), in the longest contiguous fragment possible, so the conserved regions are interspersed with more variable sections. In Gag, the boundaries simply reflect the boundaries of the p24 Capsid protein.

Example 9: Tailored Therapeutic Antigens

For Tailored antigens, either Gag or the most conserved part of Gag, p24 were used. For one design, the full M group 2015 alignments of 4596 sequences were used, to provide a global solution. An alignment of 189 contemporary B clade sequences were isolated in the US, or an alignment of 199 contemporary C clade sequences from Southern Africa. “Contemporary” refers to viruses that were isolated after 2004; all sequences sampled in the past decade were used to get a reasonable sampling of sequences from each region. Sequences that were not fully resolved or were incomplete were excluded. Regional subtype-specific contemporary viruses were selected because they represent good matches for populations that would be likely for initial proof of concept studies for evaluating for Tailored vaccines; by using single subtype regional sets we could limit the population diversity to enhance the potential for success of an HIV therapeutic vaccine.

These therapeutic vaccine antigens are designed for used in treatment scenarios where they could be matched, or “tailored”, to the sequenced infecting virus of the person who would receive the vaccine. 6 vaccine antigens would be manufactured, the best matched 2 or 3 from that set to the individuals infecting virus would be given to an individual to maximize matches between the vaccine and their infecting strain.

Thus full Gag for every epigraph is provided below, for manufacture of the 6 Tailored antigen solution, with p24 as bold face. Either Gag or the more conserved p24 could be used in a tailored vaccine.

Matched PTE scores and mismatched scores (number of extras), for delivery of the n best matched antigens out of a pool of m, for Gag proteins or the interior p24 region, where the n are selected to best cover each individual sequence in the target population. The target populations are the contemporary C clade infected population sampled in Southern Africa, or the contemporary B clade population sampled in the USA. The best solutions use a pool of m=6 antigens, and are bolded; these are the based on the six sequences shown above.

Epitope Coverage of Southern African C Clade Gag

n m Coverage Extras Vaccine evaluated against data set 1 1 0.39886 318.437 M Epigraph 2 2 0.57907 681.980 M Epigraph 1 1 0.59591 200.809 C Epigraph 2 2 0.70758 550.618 C Epigraph 2 3 0.71875 559.206 C Tailored 3 3 0.73852 778.603 C Tailored 2 4 0.72609 550.397 C Tailored 3 4 0.75294 805.658 C Tailored 2 5 0.73124 549.452 C Tailored 3 5 0.75975 800.337 C Tailored 2 6 0.73440 527.623 C Tailored 3 6 0.76513 767.759 C Tailored

Epitope Coverage of Southern African C Clade p24

n m Coverage Extras Vaccine evaluated against data set 1 1 0.48548 114.739 M Epigraph 2 2 0.76864 230.593 M Epigraph 1 1 0.73525 59.040 C Epigraph 2 2 0.84494 204.578 C Epigraph 2 2 0.84494 204.578 C Tailored 2 3 0.86153 205.387 C Tailored 3 3 0.88154 338.417 C Tailored 2 4 0.87000 188.307 C Tailored 3 4 0.89175 290.854 C Tailored 2 5 0.87699 187.849 C Tailored 3 5 0.89945 295.930 C Tailored 2 6 0.87996 191.538 C Tailored 3 6 0.90373 290.985 C Tailored

Epitope Coverage of USA B Clade Gag

Vaccine evaluated n m Coverage Extras against data set 1 1 0.55488 237.815 M Epigraph 2 2 0.67323 629.333 M Epigraph 1 1 0.61174 195.720 B Epigraph 2 2 0.72471 553.899 B Epigraph 2 3 0.73615 557.254 B Tailored 3 3 0.75734 786.772 B Tailored 2 4 0.74354 547.725 B Tailored 3 4 0.76973 762.524 B Tailored 2 5 0.74786 546.111 B Tailored 3 5 0.77777 779.487 B Tailored 2 6 0.75226 536.619 B Tailored 3 6 0.78669 773.000 B Tailored

Epitope Coverage of USA B Clade p24

Vaccine evaluated n m Coverage Extras against data set 1 1 0.73131 59.979 M EpiGraph 2 2 0.84204 214.296 M EpiGraph 1 1 0.76421 52.646 B EpiGraph 2 2 0.87373 201.233 B EpiGraph 2 3 0.88878 194.434 B Tailored 3 3 0.90810 297.571 B Tailored 2 4 0.89779 190.635 B Tailored 3 4 0.91754 282.386 B Tailored 2 5 0.90461 191.794 B Tailored 3 5 0.92704 285.005 B Tailored 2 6 0.90859 187.889 B Tailored 3 6 0.93556 280.704 B Tailored

Example 10: Vaccine Testing

The vaccine arms for initial testing in CMV include: 1) A single population episensus Gag antigen, central to the U.S. B clade epidemic; 2) The population episensus plus a tailored Gag protein selected to be a best match natural HIV-1 strain; 3) The population episensus plus a tailored Gag protein selected to be a best match different (and distant) natural HIV-1 strain; 4) The population episensus plus both Gag proteins from cohort 2 and 3. The resulting immune responses are analyzed using overlapping 15-mer peptides (4 amino acid overlap) corresponding to the vaccine antigen (to determine the total vaccine-elicited Gag-specific responses) and then to both the “target” HIV-1 strain and selected non-target HIV strains (see below) to measure the strain-specific responses and the level of epitope matching (comparing target vs. non-target HIV Gag sequences). It is determined whether computationally designed inserts and vector combinations provide higher magnitude and broader T cell responses to the target strain, while minimizing non-target strain matched responses. The results of this analysis allow for experimentally testing the predictions for epitope matching generated in Example 1.

Four cohorts of 5 Rhesus Macaques (RM) are inoculated with 10⁶ PFU of HCMV vectors as follows: cohort 1 receives a single vector containing the clade B episensus sequence, cohort 2 receives the episensus vector plus a single tailored vaccine vector, cohort 3 receives the episensus plus a different tailored vaccine vector and cohort 4 receives the episensus plus both tailored vaccine vectors. The vaccines are “tailored” towards 2 representative transmitted/founder HIV strains selected from a small set of 9 B clade U.S. HIV infections that were extensively sequenced longitudinally; this panel represents a spectrum of natural infection comparable to what might be encountered in a human clinical trial. In essence, these 2 divergent natural strains are being used as prototype patients. The episensus/tailored vaccine insert pair that is optimized for one will be suboptimal for the other and vice versa, allowing—via determination of cross-recognition to peptide sets reflecting each strain's specific sequence—reciprocal analysis of whether there is a benefit to our tailored sequence matching strategy. In addition to this reciprocal analysis, the sequence from the other 7 infections will vary in their relative “match” to the selected vaccine vector insert(s), and by analyzing the responses to peptide sets reflecting the sequences of these other 7 HIV strains, the relationship between vector insert sequence and vector combination on magnitude and breadth of matched vs. unmatched responses will be comprehensively analyzed. Part of the motivation for focusing on this set of 9 strains is that carefully sequenced full length genomes are available from longitudinal samples. The isolates are also available as infectious molecular clones, which may be useful for assessing responses in subsequent human studies. Thus by doing the groundwork in RM using this set, there will be a particularly useful matched set of proteins for direct comparisons between macaque and human responses when these vaccines are advanced into human studies.

Rhesus monkeys (RM) are inoculated subcutaneously at day 0 and week 12 and followed longitudinally for one year. Since vaccination by HCMV-vectors is not affected by pre-existing anti-RhCMV immunity, animals naturally infected with RhCMV are used for these experiments. Flow cytometric intracellular cytokine analysis (ICS) is used to determine the CD4+ and CD8+ T cell response to individual consecutive 15mer peptides comprising the vaccine sequences within the vaccine inserts administered to each animal (which will comprise the total vaccine-elicited responses). It is then determined whether these epitope-specific T cells recognize epitope variants in both the target strain and the other 8 non-target strains. For peptides that show responses to strain-specific epitopes, the magnitude, functional avidity, and functional characteristics (IFN-γ, TNF-α, IL-2 and MIP-1β production and CD107 externalization) of these responses to the “parent” (vaccine insert sequences) peptide variants are compared to determine the degree of functional cross-reactivity. In selected cases, truncation analysis is used to identify the core epitope for similar comparative analysis. To determine the percentage of MHC-II restricted CD8+ T cells present, “blocking” mAbs specific for MHC-I and MHC-II, and the invariant chain-derived, MHC-II-specific binding peptide CLIP is used to inhibit influenza-specific CD8+ T cell responses in PBMC, as has been done previously for SIV responses.

Regardless of the T cell priming results related to vector-specific genes, the immunological analysis allows for testing the hypothesis that T cells induced by tailored vaccines are superior with respect to epitope coverage of a given natural HIV reservoir compared to non-tailored vaccines. The tailored 2 antigen cocktail could likely generate T cell responses with at least 25% higher frequency of cross-reactive responses to its matched natural strain compared to episensus alone (Table 5). Testing is also done to determine whether inclusion of 3 complementary tailored antigens in a cocktail induces more cross-reactivity, or if antigen dilution or the presence of greater numbers of vaccine-specific epitopes necessitated by adding 3 instead of 2 antigens actually diminishes the magnitude or breadth of the cross reactive response to the natural protein. CMV-based T cell responses are expected to be much broader and therefore cover a much higher percentage of sequences than reported previously for other vectors. Thus, even with a relatively small number of animals there should be sufficient epitope responses to evaluate the impact of sequence variation on the cross-reactive potential of the responses. The number and magnitude of all responses to the vaccines is determined by using vaccine-matched sets of peptides. Once the targeted peptides are determined, using just those peptides that are positive in each animal, the impact of natural variation on each vaccine-responsive peptide is determined. The natural variants that are tested are based on the variation found in a reference panel, including both tailored and poorly matched Gags. Nonparametric and computational re-sampling statistical methods are used as the primary tools to evaluate the impact of epitope variation on diminishing magnitude or abrogation of recognition. These analyses are complemented, however, by using generalized linear models as needed to explore the impact of more complex interactions on T cell response cross-reactivity.

SEQ ID NO: 691  Tailored vaccine antigen episensus sequence.        MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQKIEVKDTKEALDKIEEEQNKSKKKAQQ-------AAADTGNSS---------  QVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSI  LDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKA  RVLAEAMSQVTN--SATIMMQRGNFRNQRKTVKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDC-- TERQANFLGKIWPSH-KGRPGNFLQ--------SRPEPT--------APPEESFRFGEETTTPS------------- QKQEPIDKE-----LYP-LASLRSLFGNDPSSQ  SEQ ID NO: 692  Tailored vaccine antigen 1 sequence.        MGARASVLSGGELDKWEKIRLRPGGKKKYRLKHIVWASRELERFAVNPGLLETSGGCRQILEQLQPSLQTGS  EELRSLYNTVATLYCVHQKIDVKDTKEALEKIEEEQNKSKKKAQQ-----AAAAADTGNNS---------  QVSQNYPIVQNMQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNSVGGHQAAMQMLKET  INEEAAEWDRVHPVHAGPVAPGQMREPRGSDIAGTTSTLQEQIGWMTSNPPIPVGEIYKRWIIMGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVLAEAMSQATN--SATIMMQKGNFRNQRKIVKCFNCGKEGHIAKNCRAPRKKGCWKCGREGHQMKDC-- TERQVNFLGKIWPSH-KGRPGNFLQ--------NRPEPT-----------APPAESFRFGEETTTPP---------- QKQEPIDKE-----LYP-LASLKSLFGNDPSSQ  SEQ ID NO: 693  Tailored vaccine antigen 2 sequence.        MGARASVLSGGKLDKWEKIRLRPGGKKRYKLKHIVWASRELERFAVNPGLLETAEGCRQILGQLQPALQTGS  EELKSLFNTVATLYCVHQRIDVKDTKEALEKIEEEQNKSKKKAQP------AAADTGSSS---------- QVSQNYPIVQNMQGQMVHQAISPRTLNAWVKVIEEKAFSPEVIPMFAALSEGATPQDLNLMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPVAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKKWIIMGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVLAEAMSQATN--SATIMMQRGNFKNQRKTIKCFNCGKEGHIAKNCRAPRKKGCWKCGREGHQMKDC--  TERQANFLGKIWPSH-KGRPGNFLQ--------NRPEPT-----------APPAESFRFGEETTTPP----------  QKQEPIDKE-----LYP-LASLKSLFGNDPSSQ  SEQ ID NO: 694  Tailored vaccine antigen 3 sequence.        MGARASVLSGGELDKWEKIRLRPGGKKKYRLKHIVWASRELERFAVNPGLLETAEGCRQILEQLQPSLQTGS  EELRSLYNTVAVLYCVHQRIDVKDTKEALDKIEEEQNKSKKKAQQ-----AAAAADTGNNS--------- QVSQNYPIVQNLQGQMVHQPISPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDT  INEEAAEWDRLHPVQAGPIAPGQIREPRGSDIAGTTSTLQEQIAWMTNNPPIPVGDIYKRWIILGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFYKTLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVLAEAMSQATN--SATIMMQKGNFRNQRKIVKCFNCGKEGHIAKNCRAPRKKGCWKCGREGHQMKDC--  TERQANFLGKIWPSS-KGRPGNFLQ--------SRPEPT-----------APPAESFRFGEETTTPP----------  QKQEPIDKE-----LYP-LASLKSLFGNDPSSQ  SEQ ID NO: 695  Tailored vaccine antigen 4 sequence.        MGARASVLSGGKLDKWEKIRLRPGGKKKYRLKHIVWASRELERFAVNPGLLETAEGCRQILGQLQPALQTGS  EELRSLYNTVATLYCVHQRIEVKDTKEALEKIEEEQNKSKKKVQQ------AAAADTGNSN--------- QVSQNYPIVQNIQGQMVHQPLSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTIGGHQAAMQMLKET  INEEAADWDRLHPVHAGPVAPGQMREPRGSDIAGTTSNLQEQIGWMTSNPPIPVGEIYKRWIIMGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFYKTLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVLAEAMSQVTN--SATIMMQKGNFRNQRKIVKCFNCGKEGHIAKNCRAPRKRGCWKCGKEGHQMKEC--   TERQANFLGKIWPSY-KGRPGNFLQ--------SRPEPS-----------APPEESFRFGEETATPS---------- QKQEPIDKE-----LYP-LASLKSLFGNDPSSQ  SEQ ID NO: 696  Tailored vaccine antigen 5 sequence.        MGARASVLSGGKLDKWEKIRLRPGGKKQYKLKHLVWASRELERFAVNPGLLETAEGCRQILGQLQPALQTGS  EELKSLFNTVATLYCVHQRIDVKDTKEALEKIEEEQNKSKKKAQQ-------AAADTGNNS--------- QVSQNYPIVQNIQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFSALAEGATPQDLNTMLNTVGGHQAAMQILKET  INEEAAEWDRLHPVQAGPVAPGQMREPRGSDIAGTTSNLQEQIAWMTHNPPIPVGETYKRWIIMGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFYKTLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVLAEAMSQATN--PATIMMQRGNFKNQRKIVKCFNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKEC-- TERQANFLGKIWPSY-KGRPGNFLQ--------SRPEPS-----------APPEESFRFGEETTTPP---------- QKQEPIDKE-----LYP-LTSLRSLFGNDPSSQ  SEQ ID NO: 697  EpiGraph antigen 1 sequence.        MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQRIDVKDTKEALDKIEEEQNKSKKKAQQ-------AAADTGNSS--------- QVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGETYKRWIILGLNKIVRMYSPTSI  LDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKA  RVLAEAMSQVTN--SATIMMQRGNFRNQRKTVKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDC-- TERQANFLGKIWPSH-KGRPGNFLQ--------SRPEPT-----------APPEESFRFGEETTTPS---------- QKQEPIDKE-----LYP-LASLRSLFGNDPSSQ  SEQ ID NO: 698  Epigraph cocktail antigen 1.        MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQRIDVKDTKEALDKIEEEQNKSKKKAQQ-------AAADTGNSS--------- QVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGETYKRWIILGLNKIVRMYSPTSI  LDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKA  RVLAEAMSQVTN--SATIMMQRGNFRNQRKTVKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDC-- TERQANFLGKIWPSH-KGRPGNFLQ--------SRPEPT-----------APPEESFRFGEETTTPS---------- QKQEPIDKE-----LYP-LASLRSLFGNDPSSQ  SEQ ID NO: 699  Epigraph cocktail antigen 2.        MGARASVLSGGKLDKWEKIRLRPGGKKKYRLKHIVWASRELERFALNPGLLETAEGCRQILGQLQPALQTGS  EELKSLFNTVATLYCVHQKIDVKDTKEALEKIEEEQNKSKKKAQQ-----AAAAADTGNNS--------- QVSQNYPIVQNMQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNLMLNTVGGHQAAMQILKET  INEEAADWDRLHPVHAGPVAPGQMREPRGSDIAGTTSNLQEQIGWMTSNPPIPVGETYKRWIIMGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRVYKTLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVLAEAMSQATN--SATIMMQKGNFRNQRKIVKCFNCGKEGHIAKNCRAPRKRGCWKCGKEGHQMKEC-- TERQANFLGKIWPSY-KGRPGNFLQ--------NRPEPT-----------APPAESFRFGEETTTPP---------- QKQEPIDKE-----LYP-LASLKSLFGNDPSSQ  SEQ ID NO: 700  Epigraph cocktail antigen 3.        ARASVLSGGELDKWEKIRLRPGGKKQYKLKHLVWASRELERFAINPGLLETSGGCRQILEQLQPSLQTGSEE  LRSLYNTVAVLYCVHQRIEVKDTKEALEKVEEEQNKSKKKVQQ-------AAADTGNSN--------- QVSQNYPIVQNIQGQMVHQPISPRTLNAWVKVIEDKAFSPEVIPMFAALSEGATPQDLNTMLNTIGGHQAAMQMLKDT  INEEAAEWDRLHPVQAGPVAPGQMRDPRGSDIAGTTSTLQEQIAWMTNNPPIPVGDIYKRWIILGLNKIVRMYSPTSI  LDIKQGPKESFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCRTILKALGPAATLEEMMSACQGVGGPSHKA  RILAEAMSQVTN--  STAIMMQRGNFKNQRKTVKCFNCGREGHIAKNCRAPRKKGCWKCGREGHQMKDCTERQRQANFLGKIWPSS-  KGRPGNFLQ--------SRPEPS-----------APPEESFRFGEETATPS----------QKQEPIDKE----- LYP-LTSLRSLFGNDPSLQ  SEQ ID NO: 701  HIV B gag/nef fusion Epigraph 1        MGARASVLSGGELDRWEKIRLRPGGKKKYRLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQRIEVKDTKEALDKIEEEQNKSKKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPR  TLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQM  REPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRA  EQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFR  NQRKTVKCFNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPEES  FRFGEETTTPSQKQEPIDKELYPLASLKSLFGNDPSSQGGKWSKSSIVGWPAVRERMRRAEPAAEGVGAVSRDLEKHG  AITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQG  YFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHMARE  LHPEYYKDC  SEQ ID NO: 702  HIV B gag/nef fusion Epigraph 2        MGARASVLSGGKLDKWEKIRLRPGGKKKYKLKHIVWASRELERFALNPGLLETSEGCKQILGQLQPALQTGS  EELRSLYNTVAVLYCVHQRIDVKDTKEALEKIEEEQNKCKKKAQQAAAAADTGNNSQVSQNYPIVQNMQGQMVHQALS  PRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTIGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPVAPG  QMREPRGSDIAGTTSNLQEQIAWMTHNPPIPVGEIYKRWIIMGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFYRTL  RAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQVTNSATIMMQKGN  FRNQRKIVKCFNCGKEGHIARNCRAPRKRGCWKCGKEGHQMKECTERQANFLGKIWPSYKGRPGNFLQNRPEPTAPPA  ESFRFGEETATPPQKQEPIDKEMYPLASLRSLFGNDPSQGGKWSKRSVPGWNTIRERMRRTEPAAEGVGAASRDLERH  GAITSSNTAANNAACAWLEAQEDEEVGFPVKPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQKRQEILDLWVYNTQ  GYFPDWHNYTPGPGTRFPLTFGWCFKLVPVDPEQVEKANEGENNCLLHPMSLHGMDDPEREVLVWKFDSRLAFHHVAR  EKHPEYYKDC  SEQ ID NO: 703  HIV B pol Epigraph 1        MFFRENLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAGADRQGTVSFSFPQITLWQRPLVTIKI  GGQLKEALLADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCT  LNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLV  DFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGW  KGSPAIFQSSMTKILEPFRKQNPDIVIYQLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELH  PDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVKQLCKLLRGTKALTEVVPLTEEAELELAENREILKEP  VHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKIATESIVIWGKTPKFKL  PIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTDRGRQKVVSL  TDTTNQKTQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVD  KLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLCTHL  EGKIILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTIHTNGSNFTSTTVKAACWWAGIKQEFGIPYNPQS  QGVVSMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVY  YRDSRDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED  SEQ ID NO: 704  HIV B pol Epigraph 2        MFFREDLAFPQGEAREFPSEQTRANSPTSRELQVWGGDNNSPSEAGADRQGTVSLSFPQITLWQRPLVTVKI  GGQLKEALLADDTVLEEMSLPGKWKPKMIGGIGGFIKVRQYDQVPIEICGHKTIGTVLIGPTPVNIIGRNLLTQLGCT  LNFPISPIETVPVKLKPGMDGPRVKQWPLTEEKIKALIEICTEMEKEGKISRIGPENPYNTPIFAIKKKDSTKWRKLV  DFRELNKKTQDFWEVQLGIPHPSGLKKKKSVTVLDVGDAYFSVPLDKEFRKYTAFTIPSTNNETPGIRYQYNVLPQGW  KGSPAIFQCSMTKILEPFRKQNPEIVIYQLYVGSDLEIGQHRAKIEELRQHLLKWGFTTPDKKHQKEPPFLWMGYELH  PDKWTVQPIELPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGAKALTEVIPLTKEAELELAENREILREP  VHGVYYDPTKDLIAEIQKQGLGQWTYQIYQEPFKNLKTGKYARTRGAHTNDVRQLTEAVQKITTESIVIWGKTPKFRL  PIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDGASNRETKLGKAGYVTNRGRQKVISL  TDTTNQKTLQAIYLALQDSGSEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLINKEKVYLAWVPAHKGIGGNEQV  DKLVSTGIRKVLFLDGIDRAQEEHEKYHNNWRAMASDFNLPPIVAKEIVASCDKCQLKGEAIHGQVDCSPGIWQLCTH  LEGKVILVAVHVASGYIEAEVIPAETGQETAYFILKLAGRWPVKTVHTNGSNFTSATVKAACWWAGVKQEFGIPYNPQ  SQGVVSMNNELKKIIGQIRDQAEHLKTAVQMAVFIHNFKRKGGIGEYSAGERIIDIIATDIQTRELQKQITKIQNFRV  YYRDNRDPLWKGPAKLLWKGEGAVVIQDNSEIKVVPRRKVKIIRDYGKQMAGDDCVAGRQDED  SEQ ID NO: 705  HIV M gag/nef fusion Epigraph 1        MGARASVLSGGKLDAWEKIRLRPGGKKKYRLKHLVWASRELERFALNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQRIEVKDTKEALDKIEEEQNKSKKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPR  TLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQM  REPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFYKTLRA  EQATQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFK  GQKRIKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPEESF  RFGEETTTPSQKQEPIDKELYPLASLKSLFGNDPLSQGGKWSKSSIVGWPAVRERMRRAEPAAEGVGAVSRDLEKHGA  ITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGY  FPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHMAREL  HPEYYKDC  SEQ ID NO: 706  HIV M gag/nef conserved Epigraph 1        MPIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKE  TINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVS  ILDIRQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHK  ARVLVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTF  GWCFKLVP  SEQ ID NO: 707  HIV M gag/nef fusion Epigraph 2        MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETAEGCQQIIEQLQSTLKTGS  EELKSLFNTVAVLYCVHQRIDVKDTKEALEKIEEEQNKSQQKTQQAAAGTGSSSKVSQNYPIVQNAQGQMVHQPLSPR  TLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNMMLNIVGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPIPPGQM  REPRGSDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPTSILDIKQGPKEPFRDYVDRFFKTLRA  EQASQEVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQVTNSATIMMQRGNFR  NQRKTVKCFNCGKEGHLARNCRAPRKKGCWKCGREGHQMKDCNERQANFLGKIWPSNKGRPGNFPQSRPEPTAPPAES  FRFEETTPAPKQEPKDREPLTSLKSLFGSDPLSQGSKWSKSSIVGWPAIRERMRRTEPAAEGVGAASRDLERHGAITS  SNTAANNADCAWLEAQEDEEVGFPVKPQVPLRPMTYKAAFDLSFFLKEKGGLDGLIYSQKRQDILDLWVYNTQGFFPD  WQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMDDPEREVLMWKFDSSLARRHMARELHPE  FYKDC  SEQ ID NO: 708  HIV M gag/nef conserved Epigraph 2        MPIVQNAQGQMVHQPLSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNMMLNIVGGHQAAMQMLKD  TINEEAAEWDRVHPVHAGPIPPGQMREPRGSDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPTS  ILDIKQGPKEPFRDYVDRFFKTLRAEQASQEVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPSHK  ARVLVGFPVKPQVPLRPMTYKAAFDLSFFLKEKGGLDGLIYSQKRQDILDLWVYNTQGFFPDWQNYTPGPGVRYPLTF  GWCFKLVP  SEQ ID NO: 709  HIV M pol Epigraph 1        MFFRENLAFPQGEAREFSSEQTRANSPTRRELQVWGRDNNSLSEAGADRQGTVSFSFPQITLWQRPLVTIKI  GGQLKEALLADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAIGTVLVGPTPVNIIGRNLLTQIGCT  LNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLV  DFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGW  KGSPAIFQSSMTKILEPFRKQNPDIVIYQLYVGSDLEIGQHRTKIEELRQHLLKWGFTTPDKKHQKEPPFLWMGYELH  PDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVKQLCKLLRGAKALTDIVPLTEEAELELAENREILKEP  VHGVYYDPSKDLIAEIQKQGQDQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKIATESIVIWGKTPKFRL  PIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTDRGRQKVVSL  TETTNQKTLQAIHLALQDSGSEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLSWVPAHKGIGGNEQV  DKLVSSGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPIVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLCTH  LEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKVIHTNGSNFTSAAVKAACWWAGIKQEFGIPYNPQ  SQGVVSMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKELQKQITKIQNFRV  YYRDSRDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVAGRQDEDQ  SEQ ID NO: 710  HIV M pol conserved Epigraph 1        MPQITLWQRPLVTIKIGGQLKEALLADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAIGT  VLVGPTPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPE  NPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTAFT  IPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQLYVGSDLEIGQHRTKIEELRQHLLKWG  FTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYHGQVDCSPGIWQLCTHLEGK  VILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKVIHTNGSNFTSAAVKAACWWAGIKQEFGIPYNPQSQGV  VSMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKLQKQITKIQNFRVYYRDS  RDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVAGRQDEDQ  SEQ ID NO: 711  HIV M pol Epigraph 2        MFFREDLAFPQGKAREFPSEQTRANSPTRGELQVWGGDNNSPSEAGADRQGTVSFSFPQITLWQRPLVSIKV  GGQIKEALLADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAIGTVLIGPTPVNIIGRNMLTQLGCT  LNFPISPIDTVPVTLKPGMDGPRVKQWPLTEEKIKALTEICKEMEKEGKITKIGPENPYNTPIFAIKKKDSTKWRKLV  DFRELNKKTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDESFRKYTAFTIPSTNNETPGIRYQYNVLPQGW  KGSPAIFQCSMTKILEPFRIKNPEIVIYQLYVGSDLEIGQHRAKIEELREHLLRWGFTTPDKKHQKEPPFLWMGYELH  PDRWTVQPIELPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKTP  VHGVYYDPSKDLVAEIQKQGQGQWTYQIYQEPYKNLKTGKYARKRSAHTNDVRQLTEVVQKIATESIVIWGKTPKFKL  PIQKETWEAWWTDYWQATWIPDWEFVNTPPLVKLWYQLEKDPIVGAETFYVDGAASRETKLGKAGYVTNRGRQKVVSL  TDTTNQKTLHAIHLALQDSGLEVNIVTDSQYALGIIQAQPDRSESEVVNQIIEELIKKEKVYLAWVPAHKGIGGNEQV  DKLVSAGIRKVLFLDGIDKAQEEHERYHSNWRTMASDFNLPPVVAKEIVANCDKCQLKGEAIHGQVDCSPGMWQLCTH  LEGKIILVAVHVASGYMEAEVIPAETGQETAYFILKLAGRWPVKTIHTNGSNFTSTTVKAACWWAGIQQEFGIPYNPQ  SQGVVSMNNELKKIIGQVREQAEHLKTAVQMAVFIHNFKRRGGIGGYSAGERIVDIIATDIQTRELQKQIIKIQNFRV  YYRDSRDPLWKGPAKLLWKGEGAVVIQDNSEIKVVPRRKVKIIKDYGKQMAGDDCVASRQDED  SEQ ID NO: 712  HIV M pol conserved Epigraph 2        MPQITLWQRPLVSIKVGGQIKEALLADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAIGT  VLIGPTPVNIIGRNMLTQLGCTLNFPISPIDTVPVTLKPGMDGPRVKQWPLTEEKIKALTEICKEMEKEGKITKIGPE  NPYNTPIFAIKKKDSTKWRKLVDFRELNKKTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDESFRKYTAFT  IPSTNNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRIKNPEIVIYQLYVGSDLEIGQHRAKIEELREHLLRWG  FTTPDKKHQKEPPFLWMGYELHPDRWTVQPIELPEKDSWTVNDIQKLVGKLNWASQIYHGQVDCSPGMWQLCTHLEGK  IILVAVHVASGYMEAEVIPAETGQETAYFILKLAGRWPVKTIHTNGSNFTSTTVKAACWWAGIQQEFGIPYNPQSQGV  VSMNNELKKIIGQVREQAEHLKTAVQMAVFIHNFKRRGGIGGYSAGERIVDIIATDIQTRLQKQIIKIQNFRVYYRDS  RDPLWKGPAKLLWKGEGAVVIQDNSEIKVVPRRKVKIIKDYGKQMAGDDCVASRQDED  SEQ ID NO: 713  SIV gag/nef hybrid        MGARGSVLSGKKTDELEKVRLRPGGRKKYMLKHIVWAARELDRFGSAESLLESKEGCQRILAVLAPLMPTGS  EDLKSLFSTVCVVWCLHAEMKVKDTEEAKKTVQSHLVVESGTAETMPAQSRPTAPPSGRGGNYPVQQIGGNYVHLPLS  PRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQHPQPAPQQGQL  REPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNILDVKQGPKEPFQSYVDRFYKSLR  AEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEMLTACQGVGGPGQKARLMAEALKDALTPGPIPFAAVQQ  RGQRKIIKCWNCGKTGHSARQCKAPRRKGCWKCGKAGHVMAKCPERQAGFLGFGPWGKKPHNFPMAQMPQGLTPTAPP  ADPAVDMLKNYMKMGKRQREKQRENRERPYKEVSEDLLHLSSLFGEDQPGGATSKRRSKPSGDLRQKLLRARGENYGR  LWGELEDGSSQSLGGLGKGLSSRSCEGQKYSQGQFMNTPWKNPAEEKEKLPYRKQNIDDVDEEDNDLVGVSVRPKVPL  RTMSYKLAIDMSHFIKEKGGLEGIYYSARRHRILDIYLEKEEGIIPDWQDYTSGPGIRYPKTFGWLWKLVPVDMSNEA  QEDDTHYLVHPAQTHQWSDPWGEVLVWKFDPLLAHTYEAFVRHPEEFGWKSGLPKEEVERRLAARGLLKMADKKETR  SEQ ID NO: 714  SIV gag/nef conserved        MPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDI  INEEAADWDLQHPQPAPQQGQLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNIL  DVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEMLTACQGVGGPGQKAR  LMVGVSVRPKVPLRTMSYKLAIDMSHFIKEKGGLEGIYYSARRHRILDIYLEKEEGIIPDWQDYTSGPGIRYPKTFGW  LWKLVP  SEQ ID NO: 715  SIV pol hybrid        MFFRAWPMGKEASQFPHGPDASGADTNCSPRGSSCGSTEELHEVGQKAERKAEGEQRETLQGGNGGFAAPQF  SLWRRPVVTAHIEGQPVEVLLADDSIVTGIELGPHYTPKIVGGIGGFINTKEYKNVEIEVLGKRIKGTIMTGDTPINI  FGRNLLTALGMSLNFPIAKVEPVKVALKPGKDGPKLKQWPLSKEKIVALREICEKMEKDGQLEEAPPTNPYNTPTFAI  KKKDKNKWRMLIDFRELNRVTQDFTEVQLGIPHPAGLAKRKRITVLDIGDAYFSIPLDEEFRQYTAFTLPSVNNAEPG  KRYIYKVLPQGWKGSPAIFQYTMRHVLEPFRKANPDVTLVQILIASDRTDLEHDRVVLQSKELLNSIGFSTPEEKFQK  DPPFQWMGYELWPTKWKLQKIELPQRETWTVNDIQKLVGVLNWAAQIYPGIKTKNLCKMIRGKMALTEGVQWTELAEA  ELEENRIILNQEQEGRYYREDKPLEATVLKNQDNQWTYKIHQGDRILKVGKYAKVKNTHTNGIRLLANVVQKIGKESI  VIWGQTPFFHLPVEREVWDQWWTDYWQATWIPDWDFVSTPPLIRLVFNLVKEPIEKEEVYYIDGSCNRNSKEGKAGYV  TDRGKEKVLVLEQATNQQALQAFLLALKDSGPKANIVTDSQYVLGIITGQPTESDSRIVAQIIEQMIKKSEVYIGWVP  AHKGLGGNQEVDRLVSQEIRQVLFLESIEPAQEDHDKYHSNIKELAFKFGLPRLVAKQIVDTCNKCQQKGEAIHGQAN  SDLGTWQMCTHLEGKIIIVAVHVASGFIEAEVIPQETGRQTALFLLKLAGRWPITHLHTNGANFASQEVKMVANWAGI  EHTFGVPYNPQSQGVVAMNHHLKNQIDRIREQANSVETIVLMAVHCMNFKRRGGIGDMTPAERLINMITTEQEIQFQQ  SKNSKFKNFRVYYREGRDQLWKGPGELLWKGEGAVILKVGTDIKVVPRRKAKIIKDYGGGKEVDSSSHMEDTGEAREV  A  SEQ ID NO: 716  SIV pol conserved        MPQFSLWRRPVVTAHIEGQPVEVLLADDSIVTGIELGPHYTPKIVGGIGGFINTKEYKNVEIEVLGKRIKGT  IMTGDTPINIFGRNLLTALGMSLNFPIAKVEPVKVALKPGKDGPKLKQWPLSKEKIVALREICEKMEKDGQLEEAPPT  NPYNTPTFAIKKKDKNKWRMLIDFRELNRVTQDFTEVQLGIPHPAGLAKRKRITVLDIGDAYFSIPLDEEFRQYTAFT  LPSVNNAEPGKRYIYKVLPQGWKGSPAIFQYTMRHVLEPFRKANPDVTLVQILIASDRTDLEHDRVVLQSKELLNSIG  FSTPEEKFQKDPPFQWMGYELWPTKWKLQKIELPQRETWTVNDIQKLVGVLNWAAQIYHGQANSDLGTWQMCTHLEGK  IIIVAVHVASGFIEAEVIPQETGRQTALFLLKLAGRWPITHLHTNGANFASQEVKMVANWAGIEHTFGVPYNPQSQGV  VAMNHHLKNQIDRIREQANSVETIVLMAVHCMNFKRRGGIGDMTPAERLINMITTEQEIQFQQSKNSKFKNFRVYYRE  GRDQLWKGPGELLWKGEGAVILKVGTDIKVVPRRKAKIIKDYGGGKEVDSSSHMEDTGEAREVA  SEQ ID NO: 717  HIV B pol epigraph1        FFRENLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAGADRQGTVSFSFPQITLWQRPLVTIKIG  GQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIG  CTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRK  LVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQ  GWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLW  MGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVKQLCKLLRGTKALTEVVPLTEEAELELAENR  EILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKIATESIVIWGK  TPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTDRGR  QKVVSLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKG  IGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPG  IWQLDCTHLEGKIILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQ  EFGIPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK  QITKIQNFRVYYRDSRDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED  SEQ ID NO: 718  HIV M gag Epigraph1       MGARASVLSGGKLDAWEKIRLRPGGKKKYRLKHLVWASRELERFALNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQRIEVKDTKEALDKIEEEQNKSKKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPR  TLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQM  REPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFYKTLRA  EQATQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFK  GQKRIKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPEESF  RFGEETTTPSQKQEPIDKELYPLASLKSLFGNDPLSQ  SEQ ID NO: 719  HIV M gag Epigraph1 Conserved        PIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKA  RVL  SEQ ID NO: 720  HIV M gag Epigraph2        MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETAEGCQQIIEQLQSTLKTGS  EELKSLFNTVAVLYCVHQRIDVKDTKEALEKIEEEQNKSQQKTQQAAAGTGSSSKVSQNYPIVQNAQGQMVHQPLSPR  TLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNMMLNIVGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPIPPGQM  REPRGSDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPTSILDIKQGPKEPFRDYVDRFFKTLRA  EQASQEVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQVTNSATIMMQRGNFR  NQRKTVKCFNCGKEGHLARNCRAPRKKGCWKCGREGHQMKDCNERQANFLGKIWPSNKGRPGNFPQSRPEPTAPPAES  FRFEETTPAPKQEPKDREPLTSLKSLFGSDPLSQ  SEQ ID NO: 721  HIV M gag Epigraph2 Conserved        PIVQNAQGQMVHQPLSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNMMLNIVGGHQAAMQMLKDT  INEEAAEWDRVHPVHAGPIPPGQMREPRGSDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFFKTLRAEQASQEVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 722  HIV M nef Epigraph1       MGGKWSKSSIVGWPAVRERMRRAEPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRP  QVPLRPMTYKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDP  REVEEANEGENNCLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHMARELHPEYYKDC  SEQ ID NO: 723  HIV M nef Epigraph1 Conserved        VGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPL  TFGWCFKLVP  SEQ ID NO: 724  HIV M nef Epigraph2        MGSKWSKSSIVGWPAIRERMRRTEPAAEGVGAASRDLERHGAITSSNTAANNADCAWLEAQEDEEVGFPVKP  QVPLRPMTYKAAFDLSFFLKEKGGLDGLIYSQKRQDILDLWVYNTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVEP  EKVEEANEGENNSLLHPMSLHGMDDPEREVLMWKFDSSLARRHMARELHPEFYKDC  SEQ ID NO: 725  HIV M nef Epigraph2 Conserved        VGFPVKPQVPLRPMTYKAAFDLSFFLKEKGGLDGLIYSQKRQDILDLWVYNTQGFFPDWQNYTPGPGVRYPL  TFGWCFKLVP  SEQ ID NO: 726  HIV M pol Epigraph1        FFRENLAFPQGEAREFSSEQTRANSPTRRELQVWGRDNNSLSEAGADRQGTVSFSFPQITLWQRPLVTIKIG  GQLKEALLDTGADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAIGTVLVGPTPVNIIGRNLLTQIG  CTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRK  LVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQ  GWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLKWGFTTPDKKHQKEPPFLW  MGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVKQLCKLLRGAKALTDIVPLTEEAELELAENR  EILKEPVHGVYYDPSKDLIAEIQKQGQDQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKIATESIVIWGK  TPKFRLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTDRGR  QKVVSLTETTNQKTELQAIHLALQDSGSEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLSWVPAHKG  IGGNEQVDKLVSSGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPIVAKEIVASCDKCQLKGEAMHGQVDCSPG  IWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKVIHTDNGSNFTSAAVKAACWWAGIKQ  EFGIPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKELQK  QITKIQNFRVYYRDSRDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVAGRQDEDQ  SEQ ID NO: 727  HIV M pol Epigraph1 Conserved        PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAI  GTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIG  PENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTA  FTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQ  HLLKWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYHGQVDCSPGIWQLD  CTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKVIHTDNGSNFTSAAVKAACWWAGIKQEFGIP  YNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKELQKQITKI  QNFRVYYRDSRDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVAGRQDEDQ  SEQ ID NO: 728  HIV M pol Epigraph2        FFREDLAFPQGKAREFPSEQTRANSPTRGELQVWGGDNNSPSEAGADRQGTVSFSFPQITLWQRPLVSIKVG  GQIKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAIGTVLIGPTPVNIIGRNMLTQLG  CTLNFPISPIDTVPVTLKPGMDGPRVKQWPLTEEKIKALTEICKEMEKEGKITKIGPENPYNTPIFAIKKKDSTKWRK  LVDFRELNKKTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDESFRKYTAFTIPSTNNETPGIRYQYNVLPQ  GWKGSPAIFQCSMTKILEPFRIKNPEIVIYQYMDDLYVGSDLEIGQHRAKIEELREHLLRWGFTTPDKKHQKEPPFLW  MGYELHPDRWTVQPIELPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENR  EILKTPVHGVYYDPSKDLVAEIQKQGQGQWTYQIYQEPYKNLKTGKYARKRSAHTNDVRQLTEVVQKIATESIVIWGK  TPKFKLPIQKETWEAWWTDYWQATWIPDWEFVNTPPLVKLWYQLEKDPIVGAETFYVDGAASRETKLGKAGYVTNRGR  QKVVSLTDTTNQKTELHAIHLALQDSGLEVNIVTDSQYALGIIQAQPDRSESEVVNQIIEELIKKEKVYLAWVPAHKG  IGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHERYHSNWRTMASDFNLPPVVAKEIVANCDKCQLKGEAIHGQVDCSPG  MWQLDCTHLEGKIILVAVHVASGYMEAEVIPAETGQETAYFILKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIQQ  EFGIPYNPQSQGVVESMNNELKKIIGQVREQAEHLKTAVQMAVFIHNFKRRGGIGGYSAGERIVDIIATDIQTRELQK  QIIKIQNFRVYYRDSRDPLWKGPAKLLWKGEGAVVIQDNSEIKVVPRRKVKIIKDYGKQMAGDDCVASRQDED  SEQ ID NO: 729  HIV M pol Epigraph2 Conserved        PQITLWQRPLVSIKVGGQIKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAI  GTVLIGPTPVNIIGRNMLTQLGCTLNFPISPIDTVPVTLKPGMDGPRVKQWPLTEEKIKALTEICKEMEKEGKITKIG  PENPYNTPIFAIKKKDSTKWRKLVDFRELNKKTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDESFRKYTA  FTIPSTNNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRIKNPEIVIYQYMDDLYVGSDLEIGQHRAKIEELRE  HLLRWGFTTPDKKHQKEPPFLWMGYELHPDRWTVQPIELPEKDSWTVNDIQKLVGKLNWASQIYHGQVDCSPGMWQLD  CTHLEGKIILVAVHVASGYMEAEVIPAETGQETAYFILKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIQQEFGIP  YNPQSQGVVESMNNELKKIIGQVREQAEHLKTAVQMAVFIHNFKRRGGIGGYSAGERIVDIIATDIQTRELQKQIIKI  QNFRVYYRDSRDPLWKGPAKLLWKGEGAVVIQDNSEIKVVPRRKVKIIKDYGKQMAGDDCVASRQDED  SEQ ID NO: 730  HIV B gag Epigraph1        MGARASVLSGGELDRWEKIRLRPGGKKKYRLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQRIEVKDTKEALDKIEEEQNKSKKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPR  TLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQM  REPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRA  EQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFR  NQRKTVKCFNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPEES  FRFGEETTTPSQKQEPIDKELYPLASLKSLFGNDPSSQ  SEQ ID NO: 731  HIV B gag Epigraph1 Conserved        PIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSI  LDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKA  RVL  SEQ ID NO: 732  HIV B gag Epigraph2        MGARASVLSGGKLDKWEKIRLRPGGKKKYKLKHIVWASRELERFALNPGLLETSEGCKQILGQLQPALQTGS  EELRSLYNTVAVLYCVHQRIDVKDTKEALEKIEEEQNKSKKRAQQAAADTGNNSQVSQNYPIVQNMQGQMVHQPISPR  TLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQILKETINEEAADWDRLHPVHAGPVAPGQM  REPRGSDIAGTTSNLQEQIGWMTSNPPIPVGEIYKRWIIMGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFYKVLRA  EQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKARILAEAMSQVTNSATIMMQKGNFR  NQRKIVKCFNCGKEGHIARNCRAPRKKGCWKCGREGHQMKDCNERQANFLGKIWPSYKGRPGNFLQNRPEPTAPPAES  FRFGEETTTPPQKQEPIDKDLYPLASLRSLFGNDPSS  SEQ ID NO: 733  HIV B gag Epigraph2 Conserved        PIVQNMQGQMVHQPISPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQILKET  INEEAADWDRLHPVHAGPVAPGQMREPRGSDIAGTTSNLQEQIGWMTSNPPIPVGEIYKRWIIMGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRFYKVLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RIL  SEQ ID NO: 734  HIV B nef Epigraph1        MGGKWSKSSIVGWPAVRERMRRAEPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRP  QVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEP  EKVEEANEGENNSLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHMARELHPEYYKDC  SEQ ID NO: 735  HIV B nef Epigraph1 Conserved        GFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLT  FGWCFKLVP  SEQ ID NO: 736  HIV B nef Epigraph2        MGGKWSKSSVVGWPAIRERMRRAEPAADGVGAASRDLERHGAITSSNTAANNAACAWLEAQEDEEVGFPVKP  QVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQKRQEILDLWVYHTQGFFPDWQNYTPGPGTRFPLTFGWCFKLVPVDP  DKVEEANEGENNCLLHPMSLHGMDDPEREVLVWKFDSRLAFHHVARELHPEYYKNC  SEQ ID NO: 737  HIV B nef Epigraph2 Conserved  GFPVKPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQKRQEILDLWVYHTQGFFPDWQNYTPGPGTRFPLTFGWCFK  LVP  SEQ ID NO: 738  HIV B pol Epigraph1        FFRENLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAGADRQGTVSFSFPQITLWQRPLVTIKIG  GQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIG  CTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRK  LVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQ  GWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLW  MGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVKQLCKLLRGTKALTEVVPLTEEAELELAENR  EILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKIATESIVIWGK  TPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTDRGR  QKVVSLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKG  IGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPG  IWQLDCTHLEGKIILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQ  EFGIPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK  QITKIQNFRVYYRDSRDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED  SEQ ID NO: 739  HIV B pol Epigraph1 Conserved        PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQIPIEICGHKAI  GTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIG  PENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRKYTA  FTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQ  HLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYHGQVDCSPGIWQLD  CTHLEGKIILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQEFGIP  YNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKI  QNFRVYYRDSRDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED  SEQ ID NO: 740  HIV B pol Epigraph2        FFREDLAFPQGEAREFPSEQTRANSPTRGELQVWGGDNNSPSEAGADRQGTVSLSFPQITLWQRPLVTIKVG  GQLKEALLDTGADDTVLEDMNLPGKWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLIGPTPVNIIGRNLLTQLG  CTLNFPISPIDTVPVKLKPGMDGPRVKQWPLTEEKIKALIEICTEMEKEGKISRIGPENPYNTPIFAIKKKDSTKWRK  LVDFRELNKKTQDFWEVQLGIPHPSGLKKKKSVTVLDVGDAYFSVPLDKEFRKYTAFTIPSTNNETPGIRYQYNVLPQ  GWKGSPAIFQCSMTKILEPFRKQNPEIVIYQYMDDLYVGSDLEIEQHRTKIEELRQHLLKWGFTTPDKKHQKEPPFLW  MGYELHPDKWTVQPIMLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGAKALTEVIPLTEEAELELAENR  EILREPVHGVYYDPTKDLIAEIQKQGLGQWTYQIYQEPYKNLKTGKYARTRGAHTNDVRQLTEAVQKITTESIVIWGK  TPKFRLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDGAANRDTKLGKAGYVTNKGR  QKVVTLTDTTNQKTELQAIYLALQDSGSEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKIYLAWVPAHKG  IGGNEQVDKLVSSGIRKVLFLDGIDKAQEDHEKYHSNWKAMASDFNLPPIVAKEIVASCDKCQLKGEAIHGQVDCSPG  IWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFILKLAGRWPVKTVHTDNGSNFTSATVKAACWWAGVKQ  EFGIPYNPQSQGVVESMNNELKKIIGQIRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTRELQK  QITKIQNFRVYYRDNRDPLWKGPAKLLWKGEGAVVIQDNSEIKVVPRRKVKIIRDYGKQMAGDDCVAGRQDED  SEQ ID NO: 741  HIV B pol Epigraph2 Conserved        PQITLWQRPLVTIKVGGQLKEALLDTGADDTVLEDMNLPGKWKPKMIGGIGGFIKVRQYDQILIEICGHKAI  GTVLIGPTPVNIIGRNLLTQLGCTLNFPISPIDTVPVKLKPGMDGPRVKQWPLTEEKIKALIEICTEMEKEGKISRIG  PENPYNTPIFAIKKKDSTKWRKLVDFRELNKKTQDFWEVQLGIPHPSGLKKKKSVTVLDVGDAYFSVPLDKEFRKYTA  FTIPSTNNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPEIVIYQYMDDLYVGSDLEIEQHRTKIEELRQ  HLLKWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIMLPEKDSWTVNDIQKLVGKLNWASQIYHGQVDCSPGIWQLD  CTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFILKLAGRWPVKTVHTDNGSNFTSATVKAACWWAGVKQEFGIP  YNPQSQGVVESMNNELKKIIGQIRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTRELQKQITKI  QNFRVYYRDNRDPLWKGPAKLLWKGEGAVVIQDNSEIKVVPRRKVKIIRDYGKQMAGDDCVAGRQDED  SEQ ID NO: 742  HIV C gag Epigraph1        MGARASILRGGKLDKWEKIRLRPGGKKHYMLKHLVWASRELERFALNPGLLETSEGCKQIMKQLQPALQTGT  EELRSLYNTVATLYCVHEKIEVRDTKEALDKIEEEQNKSQQKTQQAKAADGKVSQNYPIVQNLQGQMVHQAISPRTLN  AWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPIAPGQMREP  RGSDIAGTTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFFKTLRAEQA  TQDVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQANSNIMMQRSNFKGPKRI  VKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPAEPTAPPA  ESFRFEETTPAPKQEPKDREPLTSLKSLFGSDPLSQ  SEQ ID NO: 743  HIV C gag Epigraphl Conserved        PIVQNLQGQMVHQAISPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDT  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRFFKTLRAEQATQDVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPGHKA  RVL  SEQ ID NO: 744  HIV C gag Epigraph2        MGARASVLRGEKLDKWERIRLRPGGKKRYMLKHIVWASRELEKFALNPGLLETAEGCKQIIKQLHPALQTGT  EELKSLFNTVATLYCVHKKIDVRDTKEALDKIEEEQNKCQQKTQQAEAADKGKVSQNYPIVQNLQGQMVHQALSPRTL  NAWVKVVEEKAFSPEIIPMFTALSEGATPTDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPVAPGQMRE  PRGSDIAGTTSNLQEQIAWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFFKVLRAEQ  ATQEVKNWMTETLLVQNANPDCKTILRALGPGASLEEMMTACQGVGGPSHKARVLAEAMSQANNANIMMQRSNFKGSK  RIVKCFNCGKEGHIAKNCRAPRKKGCWKCGREGHQMKDCNERQANFLGKIWPSNKGRPGNFLQNRPEPTAPPAEPTAP  PAESFKFEETTPAPKQESKDREPLISLKSLFGNDPLSQ  SEQ ID NO: 745  HIV C gag Epigraph2 Conserved        PIVQNLQGQMVHQALSPRTLNAWVKVVEEKAFSPEIIPMFTALSEGATPTDLNTMLNTVGGHQAAMQMLKDT  INEEAAEWDRVHPVHAGPVAPGQMREPRGSDIAGTTSNLQEQIAWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFFKVLRAEQATQEVKNWMTETLLVQNANPDCKTILRALGPGASLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 746  HIV C nef Epigraph1        MGGKWSKSSIVGWPAVRERIRRTEPAAEGVGAASQDLDKYGALTSSNTAHNNADCAWLQAQEEEEEVGFPVR  PQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVD  PREVEEANEGENNCLLHPMSQHGMEDEDREVLKWQFDSSLARRHMARELHPEYYKDC  SEQ ID NO: 747  HIV C nef Epigraph1 Conserved        GFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGVRYPLT  FGWCFKLV  SEQ ID NO: 748  HIV C nef Epigraph2        MGSKWSKSSIVGWPAVRERMRRAEPAAEGVGAASRDLDKHGALTSSNTPANNADCAWLEAQEEEGEVGFPVK  PQVPLRPMTYKGAFDLGFFLKEKGGLDGLIYSKKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVD  PSEVEEANKGENNCLLHPMSLHGMEDEHREVLKWKFDSSLARRHLAREKHPEFYKDC  SEQ ID NO: 749  HIV C nef Epigraph2 Conserved        GFPVKPQVPLRPMTYKGAFDLGFFLKEKGGLDGLIYSKKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLT  FGWCYKLV  SEQ ID NO: 750  HIV C pol Epigraph1        FFRENLAFPQGEAREFPSEQTRANSPTSRANSPTSRELQVRGDNPRSEAGAERQGTLNFPQITLWQRPLVSI  KVGGQIKEALLDTGADDTVLEEINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAIGTVLVGPTPVNIIGRNMLT  QLGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALTAICEEMEKEGKITKIGPENPYNTPVFAIKKKDSTK  WRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEGFRKYTAFTIPSINNETPGIRYQYNV  LPQGWKGSPAIFQSSMTKILEPFRAQNPEIVIYQYMDDLYVGSDLEIGQHRAKIEELREHLLKWGFTTPDKKHQKEPP  FLWMGYELHPDKWTVQPIQLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGAKALTDIVPLTEEAELELA  ENREILKEPVHGVYYDPSKDLIAEIQKQGHDQWTYQIYQEPFKNLKTGKYAKMRTAHTNDVKQLTEAVQKIAMESIVI  WGKTPKFRLPIQKETWETWWTDYWQATWIPEWEFVNTPPLVKLWYQLEKEPIAGAETFYVDGAANRETKIGKAGYVTD  RGRQKIVSLTETTNQKTELQAIQLALQDSGSEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKERVYLSWVPA  HKGIGGNEQVDKLVSSGIRKVLFLDGIDKAQEEHEKYHSNWRAMASEFNLPPIVAKEIVASCDKCQLKGEAIHGQVDC  SPGIWQLDCTHLEGKIILVAVHVASGYIEAEVIPAETGQETAYYILKLAGRWPVKVIHTDNGSNFTSAAVKAACWWAG  IQQEFGIPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKE  LQKQIIKIQNFRVYYRDSRDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKVKIIKDYGKQMAGADCVAGRQDEDQ  SEQ ID NO: 751  HIV C pol Epigraph1 Conserved        PQITLWQRPLVSIKVGGQIKEALLDTGADDTVLEEINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAI  GTVLVGPTPVNIIGRNMLTQLGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALTAICEEMEKEGKITKIG  PENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEGFRKYTA  FTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRAQNPEIVIYQYMDDLYVGSDLEIGQHRAKIEELRE  HLLKWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIQLPEKDSWTVNDIQKLVGKLNWASQIYHGQVDCSPGIWQLD  CTHLEGKIILVAVHVASGYIEAEVIPAETGQETAYYILKLAGRWPVKVIHTDNGSNFTSAAVKAACWWAGIQQEFGIP  YNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKELQKQIIKI  QNFRVYYRDSRDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKVKIIKDYGKQMAGADCVAGRQDEDQ  SEQ ID NO: 752  HIV C pol Epigraph2        FFRENLAFQQGEAREFPSEQARANSPTSRANSPTSRELQVRGDNPCSEAGAERQGTFNFPQITLWQRPLVTI  KVGGQIKEALLDTGADDTVLEDINLPGKWKPRMIGGIGGFIKVRQYDQIPIEICGKKAIGSVLVGPTPVNIIGRNLLT  QLGCTLNFPISPIETIPVKLKPGMDGPRVKQWPLTEEKIKALTEICEEMEKEGKISKIGPENPYNTPIFAIKKKDSTK  WRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDESFRKYTAFTIPSTNNETPGIRYQYNV  LPQGWKGSPAIFQSSMTRILEPFRAKNPEIVIYQYMDDLYVGSDLEIEQHRAKIEELREHLLRWGFTTPDKKHQKEPP  FLWMGYELHPDKWTVQPIQLPEKESWTVNDIQKLVGKLNWASQIYPGIKVKQLCKLLRGTKALTDIVPLTEEAELELA  ENREILREPVHGVYYDPSKELIAEIQKQGQDQWTYQIYQEPFKNLKTGKYAKRRTAHTNDVRQLTEAVQKIALESIVI  WGKIPKFRLPIQKETWEIWWTDYWQATWIPDWEFVNTPPLVKLWYQLEKEPIAGVETFYVDGAANRETKLGKAGYVTD  KGRQKIVTLTETTNQKAELQAIQLALQDSGPEVNIVTDSQYALGIIQAQPDKSESEIVNQIIEQLINKERIYLSWVPA  HKGIGGNEQVDKLVSNGIRKVLFLDGIDKAQEEHEKYHNNWRAMASDFNLPPVVAKEIVASCDQCQLKGEAMHGQVDC  SPGIWQLDCTHLEGKVILVAVHVASGYMEAEVIPAETGQETAYFILKLAGRWPVKIIHTDNGSNFTSTAVKAACWWAG  IKQEFGIPYNPQSQGVVESMNKELKKIIGQVREQAEHLKTAVQMAVFIHNFKRRGGIGGYSAGERIIDIIASDIQTKE  LQKQITKIQNFRVYYRDSRDPIWKGPAKLLWKGEGAVVLQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVAGRQDED  SEQ ID NO: 753  HIV C pol Epigraph2 Conserved        PQITLWQRPLVTIKVGGQIKEALLDTGADDTVLEDINLPGKWKPRMIGGIGGFIKVRQYDQIPIEICGKKAI  GSVLVGPTPVNIIGRNLLTQLGCTLNFPISPIETIPVKLKPGMDGPRVKQWPLTEEKIKALTEICEEMEKEGKISKIG  PENPYNTPIFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDESFRKYTA  FTIPSTNNETPGIRYQYNVLPQGWKGSPAIFQSSMTRILEPFRAKNPEIVIYQYMDDLYVGSDLEIEQHRAKIEELRE  HLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIQLPEKESWTVNDIQKLVGKLNWASQIYHGQVDCSPGIWQLD  CTHLEGKVILVAVHVASGYMEAEVIPAETGQETAYFILKLAGRWPVKIIHTDNGSNFTSTAVKAACWWAGIKQEFGIP  YNPQSQGVVESMNKELKKIIGQVREQAEHLKTAVQMAVFIHNFKRRGGIGGYSAGERIIDIIASDIQTKELQKQITKI  QNFRVYYRDSRDPIWKGPAKLLWKGEGAVVLQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVAGRQDE  SEQ ID NO: 754  HIV M Gag episensus EG-0, Tailored        MGARASVLSGGKLDAWEKIRLRPGGKKKYRLKHLVWASRELERFALNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQRIEVKDTKEALDKIEEEQNKSKKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPR  TLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQM  REPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFYKTLRA  EQATQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFK  GQKRIKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAQSRPEP  TAPPAESFRPQPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLKSLFGNDPLSQY  SEQ ID NO: 755  HIV M gag episensus EG-0 Conserved, Tailored        PIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKA  RVL  SEQ ID NO: 756  HIV M gag CEN-1, Tailored        MGARASVLTGGKLDAWERIRLRPGGKKKYRMKHLVWASRELERFAINPGLLETAEGCQQIIEQLQSTLKTGS  EELKSLFNTVATLWCVHQRIEIKDTKEALDKLEEVQNKSQQKTQQAAAGTGSSSKVSQNYPIVQNAQGQMVHQPLSPR  TLNAWVKVVEEKGFNPEVIPMFSALSDGATPQDLNMMLNIVGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPIPPGQM  REPRESDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIILGLHKIVRMYSPVGILDIKQGPKEPFRDYVDRFFKTLRA  EQASQEVKNWMTETLLIQNANPDCKSILKALGTGATLEEMMTACQGVGGPSHKARVLAEAMSQAQHANIMMQRGNFKG  QRKIKCFNCGKEGHLARNCRAPRKRGCWKCGQEGHQMKDCNERQANFLGKIWPSNKGRPGNFPQSRPEPTAPRTEPTA  PPARPEPTAPPLQSRLEPTAPPAEPTAPPAENWGMGEEITSLLKQEQKDKEHPPPLVSLKSLFGNDPLLQ  SEQ ID NO: 757  HIV M gag CEN-1 Conserved, Tailored        PIVQNAQGQMVHQPLSPRTLNAWVKVVEEKGFNPEVIPMFSALSDGATPQDLNMMLNIVGGHQAAMQMLKDT  INEEAAEWDRVHPVHAGPIPPGQMREPRESDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIILGLHKIVRMYSPVGI  LDIKQGPKEPFRDYVDRFFKTLRAEQASQEVKNWMTETLLIQNANPDCKSILKALGTGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 758  HIV M gag CEN-2, Tailored        MGARASILRGGKLDKWEKIRLRPGGKKHYMLKHIVWASRELEKFALNPDLLETSEGCKQIIKQLQPALQTGT  EELRSLFNTVATLYCVHEKIEVRDTKEALDKVEEEQNKSQQKTQQAKAADGKVSQNYPIVQNAQGQMVHQALSPRTLN  AWVKVIEEKAFSPEVIPMFTALSEGATPSDLNTMLNTIGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPVAPGQMREP  RGSDIAGSTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVKMYSPVSILDIKQGPKEPFRDYVDRFFKTLRAEQA  TQDVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQANSNIMMQRSNFKGPKRI  VKCFNCGKEGHIAKNCRAPRKKGCWKCGREGHQMKDCNERQANFLGKIWPSNKGRPGNFLQNRPEPTAPPLQSRLEPT  APLEPTAPPEPTAPPAVVPTAPPVEPTAPPAEPTAPPAESFRFEETTPAPKQEPKDREPLTSLKSLFGSDPLSQ  SEQ ID NO: 759  HIV M gag CEN-2 Conserved, Tailored        PIVQNAQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPSDLNTMLNTIGGHQAAMQMLKDT  INEEAAEWDRVHPVHAGPVAPGQMREPRGSDIAGSTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVKMYSPVSI  LDIKQGPKEPFRDYVDRFFKTLRAEQATQDVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 760  HIV M gag CEN-3, Tailored        MGSRASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCKQILGQLQPALQTGS  EELRSLYNTVAVLYCVHQRIDVKDTKEALEKIEEEQNKCKKKAQQAAAAADTGNNSQVSQNYPIVQNIQGQMVHQALS  PRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPSDLNTMLNTIGGHQAAMQMLKDTINEEAADWDRLHPVQAGPVAPG  QMRDPRGSDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIIMGLNKIVRMYSPTSILDIKQGPKEPFRDYVDRFFKTL  RAEQASQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQATNSAAIMMQRGN  FRNQRKIVKCFNCGKEGHIAKNCRAPRKRGCWKCGREGHQMKDCNERQANFLGRIWPSNKGRPGNFLQNRPEPTAPNF  LQSRPEPSAPPEPTAPPEESFRFGEETATPSQKQEPTDKELYPLASLRSLFGNDPSSQ  SEQ ID NO: 761  HIV M gag CEN-3 Conserved, Tailored        PIVQNIQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPSDLNTMLNTIGGHQAAMQMLKDT  INEEAADWDRLHPVQAGPVAPGQMRDPRGSDIAGTTSNLQEQIGWMTSNPPIPVGDIYKRWIIMGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFFKTLRAEQASQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 762  HIV M gag CEN-4, Tailored        MGTRASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETAEGCRQILEQLQPALQTGS  EELRSLYNTVAVLYCVHQRIDVKDTKEALEKIEEEQNKCKKKAQQTAADTGNNSQVSQNYPIVQNMQGQMVHQPISPR  TLNAWVKVIEEKAFSPEVIPMFTALSEGATPHDLNTMLNTIGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPVAPGQM  REPKGSDIAGTTSNLQEQIGWMTHNPPIPVGDIYKRWIIMGLNKIVRMYSPTSILDIKQGPKEPFRDYVDRFFKTLRA  EQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMSACQGVGGPSHKARILAEAMSQATNSANIMMQRGNFR  NQRKTVKCFNCGKEGHIAKNCRAPRKRGCWKCGREGHQMKDCNERQANFLGKIWPSYKGRPGNFLQNRPEPTAPPEPT  APPEESFGFGEETTTPPQKQEPIDKDLYPLASLRSLFGNDPSSQ  SEQ ID NO: 763  HIV M gag CEN-4 Conserved, Tailored        PIVQNMQGQMVHQPISPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPHDLNTMLNTIGGHQAAMQMLKDT  INEEAAEWDRVHPVHAGPVAPGQMREPKGSDIAGTTSNLQEQIGWMTHNPPIPVGDIYKRWIIMGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFFKTLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMSACQGVGGPSHKA  RIL  SEQ ID NO: 764  HIV M gag CEN-5, Tailored        MGARASILSGGKLDAWERIRLRPGGKKKYRMKHLVWASRELDRFALNPSLLETAEGCQQIMEQLQPALKTGT  EELRSLFNTVATLYCVHQRIDVKDTKEALDKIEEIQNKSKQKTQQAAADTGNSSKVSQNYPIVQNAQGQMIHQSLSPR  TLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNMMLNIVGGHQAAMQMLKDTINEEAAEWDRVHPVHAGPIPPGQM  REPRGGDIAGTTSTPQEQIGWMTSNPPIPVGDIYKRWIILGLHKLVRMYSPVSILDIKQGPKEPFRDYVDRFFKTLRA  EQATQEVKGWMTETLLIQNANPDCKSILRALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQVQHTNIMMQRGNFRG  QKRIKCFNCGKEGHLARNCRAPRKRGCWKCGREGHQMKDCNERQANFLGKIWPSSKGRPGNFPQSRPEPTAPQNRLEP  TAPPAEPTAPPAEIFGMGEEITSPPKQEQKDREQAPPLVSLKSLFGNDLLSQ  SEQ ID NO: 765  HIV M gag CEN-5 Conserved, Tailored        PIVQNAQGQMIHQSLSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNMMLNIVGGHQAAMQMLKDT  INEEAAEWDRVHPVHAGPIPPGQMREPRGGDIAGTTSTPQEQIGWMTSNPPIPVGDIYKRWIILGLHKLVRMYSPVSI  LDIKQGPKEPFRDYVDRFFKTLRAEQATQEVKGWMTETLLIQNANPDCKSILRALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 766  HIV C gag episensus EG-0, Tailored        MGARASILRGGKLDKWEKIRLRPGGKKHYMLKHLVWASRELERFALNPGLLETSEGCKQIMKQLQPALQTGT  EELRSLYNTVATLYCVHEKIEVRDTKEALDKIEEEQNKSQQKTQQAKAADGKVSQNYPIVQNLQGQMVHQAISPRTLN  AWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPVAPGQMREP  RGSDIAGTTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFFKTLRAEQA  TQDVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQANSNIMMQRSNFKGPKRI  VKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPAEPTAPPA  ESFRFEETTPAPKQEPKDREPLTSLKSLFGSDPLSQ  SEQ ID NO: 767  HIV C gag episensus EG-0 Conserved, Tailored        PIVQNLQGQMVHQAISPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDT  INEEAAEWDRLHPVHAGPVAPGQMREPRGSDIAGTTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRFFKTLRAEQATQDVKNWMTDTLLVQNANPDCKTILRALGPGATLEEMMTACQGVGGPGHKA  RVL  SEQ ID NO: 768  HIV C gag CEN-1, Tailored        MGARASILRGEKLDKWEKIKLRPGGKKRYMLKHLIWASRELERFALNPSLLETSEGCKQIIKQLQPALKTGT  EELRSLFNTVATLYCVHAGIEVRDTKEALDRIEEEQNKCQQKTQQAEAADKGKVSQNYPIVQNAQGQMVHQALSPRTL  NAWVKVVEEKAFSPEIIPMFTALSEGATPSDLNSMLNTVGGHQAAMQMLKDTINDEAAEWDRVHPVHAGPIAPGQMRE  PRGSDIAGTTSNLQEQIAWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFFKCLRAEQ  ATQEVKNWMTDTLLIQNANPDCKTILKALGPGASLEEMMTACQGVGGPSHKARVLAEAMSQVNNANIMMQRGNFKGPK  RIIKCFNCGKEGHLARNCRAPRKKGCWKCGQEGHQMKDCSNERQANFLGKLWPSHKGGRPGNFLQNRPEPTAPPVEPT  APPAEPTAPPAESFKFEETTPVPKQELKDREPLISLKSLFGNDPLSQ  SEQ ID NO: 769  HIV C gag CEN-1 Conserved, Tailored        IVQNAQGQMVHQALSPRTLNAWVKVVEEKAFSPEIIPMFTALSEGATPSDLNSMLNTVGGHQAAMQMLKDTI  NDEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSNLQEQIAWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSIL  DIRQGPKEPFRDYVDRFFKCLRAEQATQEVKNWMTDTLLIQNANPDCKTILKALGPGASLEEMMTACQGVGGPSHKAR  VL  SEQ ID NO: 770  HIV C gag CEN-2, Tailored        MGARASILRGEKLDKWERIRLRPGGKKHYMIKHLVWASRELEKFALNPGLLETADGCKQIIKQLHPALQTGT  EELKSLYNTVATLYCVHERIEVRDTKEALDRIEEEQNKCQQKTQQAEAADKGKVSQNYPIVQNAQGQMVHQPISPRTL  NAWVKVVEEKAFSPEIIPMFTALSEGATPTDLNTMLNTIGGHQAAMQILKDTINEEAVEWDRLHPVQAGPVAPGQIRE  PRGSDIAGTTSNLQEQIAWMTGNPPVPVGDIYKRWIIMGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFFKVLRAEQ  ATQEVKNWMTETLLVQNANPDCKIILKGLGPAATLEEMMTACQGVGGPSHKARVLAEAMSQVNNTNIMMQKSNFKGPK  RTVKCFNCGKEGHIAKNCRAPRKKGCWKCGREGHQMKDCNERQANFLGKIWPSQKGRPGNFLQNRPEPTAPRPEPTAP  PRLEPTAPPAEPSAPPAESFRFEGTTPAPKQESKDREPLISLKSLFGNDPLSQ  SEQ ID NO: 771  HIV C gag CEN-2 Conserved, Tailored        PIVQNAQGQMVHQPISPRTLNAWVKVVEEKAFSPEIIPMFTALSEGATPTDLNTMLNTIGGHQAAMQILKDT  INEEAVEWDRLHPVQAGPVAPGQIREPRGSDIAGTTSNLQEQIAWMTGNPPVPVGDIYKRWIIMGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFFKVLRAEQATQEVKNWMTETLLVQNANPDCKIILKGLGPAATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 772  HIV C gag CEN-3, Tailored        MGARASILRGEKLDRWERIRLRPGGKKCYMLKHIVWASRELERFSLNPGLLETAEGCKQIIKQLHPALQTGT  EELKSLFNTVATLYCVHKKIDVRDTKEALDKVEEEQNKCQQKTQQAKAADEKVSQNYPIVQNIQGQMVHQALSPRTLN  AWVKVVEEKAFSPEIIPMFTALSEEATPQDLNTMLNAVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREP  RGSDIAGTTSNLQEQIAWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFFRVLRAEQA  TQEVKNWMTDTLLIQNANPDCKTILKALGPGASLEEMMTACQGVGGPSHKARVLAEAMSQANNINIMMQRGNFKGPKR  TVKCFNCGKEGHIAKNCRAPRKRGCWKCGKEGHQMKDCNERQANFLGRIWPSHKGRPGNFLQNRPEPTAPSAESFRQN  RTEPTAPPARLEPTAPPAEPSAPPVESFRFEETTPALKQESKDREPLTSLRSLFGSDPLFQ  SEQ ID NO: 773  HIV C gag CEN-3 Conserved, Tailored        PIVQNIQGQMVHQALSPRTLNAWVKVVEEKAFSPEIIPMFTALSEEATPQDLNTMLNAVGGHQAAMQMLKET  INEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSNLQEQIAWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFFRVLRAEQATQEVKNWMTDTLLIQNANPDCKTILKALGPGASLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 774  HIV C gag CEN-4, Tailored        MGARASVLRGEKLDKWERIRLRPGGKKQYMLKHIVWASRELEKFALNPGLLETAEGCKQIIKQLHPALQTGT  EELRSLFNTVATLYCVHKGIDVRDTKEALDKVEEEQNKCQQKTQQAEADKKVSQNYPIVQNIQGQMVHQPLSPRTLNA  WVKVVEEKAFSPEVIPMFSALSEGATPGDLNTMLNTIGGHQAAMQMLKDTINDEAAEWDRLHPVHAGPIAPGQMREPR  GSDIAGTTSNLQEQIAWMTNNPPVPVGEIYKRWIVLGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFFRTLRAEQAT  QEVKNWMTETLLVQNANPDCKNILRALGPGASLEEMMTACQGVGGPSHKARVLAEAMSQANNTNIMMQKSNFKGPRRI  VKCFNCGKEGHIAKNCRAPRKRGCWKCGKEGHQMKDCNERQANFLGKIWPSNKGRPGNFLQNRPEPTAPQSRPEPTAP  LEPTAPPAEPTAPPAESFKFEETTPAPKQEQKDREPLISLKSLFGNDPLSQ  SEQ ID NO: 775  HIV C gag CEN-4 Conserved, Tailored        PIVQNIQGQMVHQPLSPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPGDLNTMLNTIGGHQAAMQMLKDT  INDEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSNLQEQIAWMTNNPPVPVGEIYKRWIVLGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFFRTLRAEQATQEVKNWMTETLLVQNANPDCKNILRALGPGASLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 776  HIV C gag CEN-5, Tailored        MGARASVLRGEKLDKWERIRLRPGGKKRYMLKHIVWASRELEKFALNPGLLETAEGCKQIIKQLQPALQTGT  EELKSLFNTVATLYCVHEKIDVRDTKEALDRIEEEQNKCQQKTQQAKAADEKVSQNYPIVQNAQGQMVHQALSPRTLN  AWVKVIEEKGFNPEVIPMFTALSDGATPQDLNSMLNTVGGHQAAMQILKDTINEEAAEWDRVHPVHAGPIAPGQMREP  RGSDIAGTTSNLQEQIAWMTGNPPIPVGEIYKRWIILGLNKIVRMYSPVSILDIRQGPKEPFRDYVDRFFKALRAEQA  TQEVKNWMTETLLVQNANPDCKNILRALGPGASLEEMMTACQGVGGPSHKARVLAEAMSQANNTNIMMQRNNFKGPKR  IIKCFNCGKEGHIAKNCRAPRKKGCWKCGREGHQMKDCNERQANFLGRIWPSHKGGRPGNFLQNRPEPTAPPVEPTAP  PAEPTAPPAESFKFEETTPTPKQEQKDREPLISLKSLFGNDPLSQ  SEQ ID NO: 777  HIV C gag CEN-5 Conserved, Tailored        PIVQNAQGQMVHQALSPRTLNAWVKVIEEKGFNPEVIPMFTALSDGATPQDLNSMLNTVGGHQAAMQILKDT  INEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSNLQEQIAWMTGNPPIPVGEIYKRWIILGLNKIVRMYSPVSI  LDIRQGPKEPFRDYVDRFFKALRAEQATQEVKNWMTETLLVQNANPDCKNILRALGPGASLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 778  HIV B gag episensus EG-0, Tailored        MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGS  EELKSLYNTVATLYCVHQKIDVKDTKEALDKIEEEQNKSKKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPR  TLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQM  REPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRA  EQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFR  NQRKTVKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPPEPT  APPEESFRFGEETTTPSQKQEPIDKELYPLASLKSLFGNDPSSQ  SEQ ID NO: 779  HIV B gag episensus EG-0 Conserved, Tailored        PIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKET  INEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSI  LDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKA  RVL  SEQ ID NO: 780  HIV B gag CEN-1, Tailored        MGSRASVLSGGKLDQWEKIRLRPGGKKRYKLKHLVWASRELERFAVNPSLLETSEGCKQILGQLQPALQTGS  EELRSLYNTIAVLYCVHQRIEVKDTKEALEKIEEEQNKCKKKAQQAAAAAADTGNSNQVSQNYPIVQNMQGQMVHQAL  SPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPADLNTMLNTIGGHQAAMQILKETINEEAAEWDRVHPVHAGPVAP  GQMREPRGSDIAGSTSTLQEQIAWMTSNPPIPVGDIYKRWIIMGLNKIVRMYSPTSILDIKQGPKEPFRDYVDRFYKT  LRAEQATQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQVTTPPAIMMQRG  NFKNQRKIVKCFNCGKEGHLARNCRAPRKRGCWKCGREGHQMKDCSERQANFLGKIWPSYKGRPGNFLQNRPEPTAPP  AEPTAPPAESFRFGEETATPPQKQEPIDKEMYPLTSLRSLFGNDPSQ  SEQ ID NO: 781  HIV B gag CEN-1 Conserved, Tailored        PIVQNMQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPADLNTMLNTIGGHQAAMQILKET  INEEAAEWDRVHPVHAGPVAPGQMREPRGSDIAGSTSTLQEQIAWMTSNPPIPVGDIYKRWIIMGLNKIVRMYSPTSI  LDIKQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 782  HIV B gag CEN-2, Tailored        MGSRASVLSGGKLDKWEKIRLRPGGKKKYRLKHLVWASRELERYALNPGLLETAEGCRQILGQLQPALQTGS  EELKSLFNTVAVLYCVHQRIDVKDTKEALEKIEEEQNKSKKKTQQAAADTGNNSQVKVSQNYPIVQNIQGQMVHQALS  PRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPHDLNTMLNTIGGHQAAMQILKETINDEAAEWDRTHPVHAGPVAPG  QMRDPRGSDIAGTTSNLQEQIGWMTHNPPIPVGDIYKRWIIMGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFYKIL  RAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQMTNSATIMMQKGN  FRNQRKTIKCFNCGKEGHLARNCRAPRKRGCWKCGQEGHQMKDCNERQANFLGKIWPSSKGRPGNFLQSRPESRPEPT  APPAEPTAPPAESFRFGEETATPPQKQEPIDKEMYPLASLRSLFGNDPSSK  SEQ ID NO: 783  HIV B gag CEN-2 Conserved, Tailored        PIVQNIQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPHDLNTMLNTIGGHQAAMQILKET  INDEAAEWDRTHPVHAGPVAPGQMRDPRGSDIAGTTSNLQEQIGWMTHNPPIPVGDIYKRWIIMGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRFYKILRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 784  HIV B gag CEN-3, Tailored        MGARASILSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFALNPGLLETSGGCRQILEQLQPALQTGS  EELRSLYNTVAVLYCVHQRIEVKDTKEALEKIEEEQNKCKKKAQQAAAAAADTGNNSQVSQNYPIVQNIQGQMVHQAL  SPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPGDLNLMLNAVGGHQAAMQMLKDTINEEAADWDRLHPVQAGPVAP  GQLREPRGSDIAGTTSNLQEQIAWMTHNPPIPVGEIYKRWIIMGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFYRT  LRAEQASQDVKNWMTETLLIQNANPDCRTILKALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQATSSATIMMQKG  NFRNQRKIVKCFNCGKEGHIAKNCRAPRKRGCWKCGREGHQMKDCSERQANFLGKIWPSYKGRPGNFLQNRPEPTAPP  AEPTAPPAESFRFGEETTTPPQKQEPTDKELYPLASLRSLFGNDPLSQ  SEQ ID NO: 785  HIV B gag CEN-3 Conserved, Tailored        PIVQNIQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPGDLNLMLNAVGGHQAAMQMLKDT  INEEAADWDRLHPVQAGPVAPGQLREPRGSDIAGTTSNLQEQIAWMTHNPPIPVGEIYKRWIIMGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRFYRTLRAEQASQDVKNWMTETLLIQNANPDCRTILKALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 786  HIV B gag CEN-4, Tailored        MGARASILSGGELDKWEKIRLRPGGKKKYRLKHIVWASNELERFALNPGLLETSDGCRQILGQLHPSLQTGS  EELRSLYNTVAVLYCVHQRIEIKDTKEALEKIEEEQNKCKKKAQQAAAAQQAAAGTGNNSQVSQNYPIVQNMQGQMVH  QALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPHDLNTMLNTIGGHQAAMQILKETINEEAAEWDRVHPVHAGP  IAPGQIREPRGSDIAGTTSNLQEQIGWMTHNPPIPVGEIYKKWIIMGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRF  YKTLRAEQATQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPAHKARVLAEAMSQATNSAAIMM  QKGNFRNQRRTVKCFNCGKEGHIAKNCRAPRKKGCWKCGQEGHQMKDCNERQANFLGRSWPSLKGRPGNFLQNRPEPS  APPEESFKFGEETTTPPQKQEPIDKDLYPLASLRSLFGNDPSST  SEQ ID NO: 787  HIV B gag CEN-4 Conserved, Tailored        PIVQNMQGQMVHQALSPRTLNAWVKVIEEKAFSPEVIPMFTALSEGATPHDLNTMLNTIGGHQAAMQILKET  INEEAAEWDRVHPVHAGPIAPGQIREPRGSDIAGTTSNLQEQIGWMTHNPPIPVGEIYKKWIIMGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPAHKA  RVL  SEQ ID NO: 788  HIV B gag CEN-5, Tailored        MGSRASVLSGGKLDKWEKIRLRPGGKKKYQLKHIVWASRELERYALNPGLLETAEGCRQILEQLQPALQTGS  EELRSLYNTVAVLYCVHQKIEVKDTKEALEKVEEEQNKSKKRIQQAQQAAAADTGNSSKVSQNYPIVRNLQGQMVHQP  ISPRTLNAWVKVIEEKAFSPEVIPMFSALAEGATPQDLNLMLNAVGGHQAAMQMLKDTINEEAAEWDRMHPVHAGPVA  PGQMREPRGSDIAGTTSNLQEQIGWMTSNPPIPVGEIYKRWIIMGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFYR  TLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKARVLAEAMSQVTNSTAIMMQR  GNFKNQRKIVKCFNCGKEGHIAKNCRAPRKRGCWKCGREGHQMKECTERQVNFLGKIWPSYKGRPGNFLQNRPEPTAP  PAPPEESFRFGEGTTTPSQKQGTIDKELYPLTSLRSLFGNDPS  SEQ ID NO: 789  HIV B gag CEN-5 Conserved, Tailored        PIVRNLQGQMVHQPISPRTLNAWVKVIEEKAFSPEVIPMFSALAEGATPQDLNLMLNAVGGHQAAMQMLKDT  INEEAAEWDRMHPVHAGPVAPGQMREPRGSDIAGTTSNLQEQIGWMTSNPPIPVGEIYKRWIIMGLNKIVRMYSPVSI  LDIKQGPKEPFRDYVDRFYRTLRAEQASQDVKNWMTETLLVQNSNPDCKTILKALGPGATLEEMMTACQGVGGPSHKA  RVL  SEQ ID NO: 790  VTSSNMNNA  SEQ ID NO: 791  TSSNMNNAD  SEQ ID NO: 792  TSSNMNNADSVWLRAQEEE  SEQ ID NO: 793  TSSNMNNADCVWLRAQEEE  SEQ ID NO: 794  ARCHSLM  SEQ ID NO: 795  DEFGKLM  SEQ ID NO: 796  EPTAPPAEPTAP  SEQ ID NO: 797  PTAPPAEPTAPP  SEQ ID NO: 798  EPTAPPAEPTAPP  SEQ ID NO: 799  ARCGSLM  SEQ ID NO: 800  ARCGSPM  SEQ ID NO: 801  ARYGSLM  SEQ ID NO: 802  AYCHSLM  SEQ ID NO: 803  YRCHSLM  SEQ ID NO: 804  DEFGSLM  SEQ ID NO: 805  DEFGKLM  SEQ ID NO: 806  ARCCDEGH  SEQ ID NO: 807  ARCDEFGH  SEQ ID NO: 808  ARCCDE-GH  SEQ ID NO: 809  ARC-DEFGH  SEQ ID NO: 810  DECHJLM  SEQ ID NO: 811  DEFGJLM  

What is claimed is:
 1. An HIV-1 vaccine comprising one or more carriers and one or more population episensus antigens determined using the EpiGraph approach.
 2. The vaccine of claim 1, wherein the carrier is DNA, protein, or a vector
 3. The vaccine of claim 2, wherein the vector is a viral vector or a bacterial vector.
 4. The vaccine of claim 3, wherein the viral vector comprises a poxvirus, adenoviruses, rubella, sendai virus, rhabdovirus, alphavirus or adeno-associated virus backbone.
 5. The vaccine of claim 3, wherein the viral vector comprises a cytomegalovirus (CMV) backbone.
 6. The vaccine of claim 5, wherein the CMV backbone is a human CMV (HCMV) backbone or a rhesus CMV (RhCMV) backbone.
 7. The vaccine of claim 5 or 6, wherein the CMV backbone lacks the UL130-128 gene region.
 8. The vaccine of any one of claims claim 5-7, wherein the CMV backbone lacks the UL82 gene encoding the tegument protein pp71.
 9. The vaccine of any one of claims 1-8, wherein the population episensus antigen comprises epitopes from Gag, Pol, Nef, Env, Tat, Rev, Vpr, Vif, or Vpu.
 10. The vaccine of claim 9, wherein the population episensus antigen comprises Gag epitopes.
 11. The vaccine of claim 10, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 697, SEQ ID NO: 698, SEQ ID NO: 699, or SEQ ID NO:
 700. 12. The vaccine of any one of claims 1-11, wherein the population episensus antigen comprises epitopes from a conserved region of HIV.
 13. The vaccine of claim 12, wherein in the population episensus antigen comprises epitopes from a conserved region of Gag, Pol, or Nef.
 14. The vaccine of claim 13, wherein the population episensus antigen comprises epitopes from the p24 capsid protein of Gag.
 15. The vaccine of any one of claims 1-14, wherein the population episensus antigen is a fusion antigen comprising two or more population episensus antigens.
 16. The vaccine of claim 15, wherein the fusion antigen comprises a population episensus antigen comprising Gag epitopes and a population episensus antigen comprising Nef epitopes.
 17. The vaccine of claim 15, wherein the fusion antigen comprises a population episensus antigen comprising epitopes from a conserved region of Gag and a population episensus antigen comprising epitopes from a conserved region of Nef.
 18. The vaccine of claim 17, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 19. The vaccine of any one of claims 1-18, wherein the population episensus antigen is central to the HIV-1 B clade epidemic in the United States.
 20. The vaccine of claim 19, wherein the population episensus antigen comprises Gag epitopes.
 21. The vaccine of claim 20, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 730 or SEQ ID NO:
 732. 22. The vaccine of claim 19, wherein the population episensus antigen comprises epitopes from a conserved region of Gag.
 23. The vaccine of claim 22, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 24. The vaccine of claim 23, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 731 or SEQ ID NO:
 733. 25. The vaccine of claim 19, wherein the population episensus antigen comprises Nef epitopes.
 26. The vaccine of claim 25, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 734 or SEQ ID NO:
 736. 27. The vaccine of claim 19, wherein the population episensus antigen comprises epitopes from a conserved region of Nef.
 28. The vaccine of claim 27, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 735 or SEQ ID NO: 737
 29. The vaccine of claim 19, wherein the population episensus antigen is a fusion antigen comprising two or more population episensus antigens.
 30. The vaccine of claim 29, wherein the fusion antigen comprises a population episensus antigen comprising Gag epitopes and a population episensus antigen comprising Nef epitopes.
 31. The vaccine of claim 30, wherein the fusion antigen comprises the amino acid sequence of SEQ ID NO: 701 or SEQ ID NO:
 702. 32. The vaccine of claim 29, wherein the fusion antigen comprises a population episensus antigen comprising epitopes from a conserved region of Gag and a population episensus antigen comprising epitopes from a conserved region of Nef.
 33. The vaccine of claim 32, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 34. The vaccine of claim 19, wherein the population episensus antigen comprises Pol epitopes.
 35. The vaccine of claim 34, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 703, SEQ ID NO: 704, SEQ ID NO: 738 or SEQ ID NO:
 740. 36. The vaccine of claim 19, wherein the population episensus antigen comprises epitopes from a conserved region of Pol.
 37. The vaccine of claim 36, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 739 or SEQ ID NO:
 741. 38. The vaccine of any one of claims 1-18, wherein the population episensus antigen is central to the HIV-1 C clade epidemic in South Africa.
 39. The vaccine of claim 38, wherein the population episensus antigen comprises Gag epitopes.
 40. The vaccine of claim 39, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 742 or SEQ ID NO:
 744. 41. The vaccine of claim 38, wherein the population episensus antigen comprises epitopes from a conserved region of Gag.
 42. The vaccine of claim 41, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 43. The vaccine of claim 42, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 743 or SEQ ID NO:
 745. 44. The vaccine of claim 38, wherein the population episensus antigen comprises Nef epitopes.
 45. The vaccine of claim 44, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 746 or SEQ ID NO:
 748. 46. The vaccine of claim 38, wherein the population episensus antigen comprises epitopes from a conserved region of Nef.
 47. The vaccine of claim 46, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 747 or SEQ ID NO:
 749. 48. The vaccine of claim 38, wherein the population episensus antigen is a fusion antigen comprising two or more population episensus antigens.
 49. The vaccine of claim 48, wherein the fusion antigen comprises a population episensus antigen comprising Gag epitopes and a population episensus antigen comprising Nef epitopes.
 50. The vaccine of claim 48, wherein the fusion antigen comprises a population episensus antigen comprising epitopes from a conserved region of Gag and a population episensus antigen comprising epitopes from a conserved region of Nef.
 51. The vaccine of claim 50, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 52. The vaccine of claim 38, wherein the population episensus antigen comprises Pol epitopes.
 53. The vaccine of claim 52, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 750 or SEQ ID NO:
 752. 54. The vaccine of claim 38, wherein the population episensus antigen comprises epitopes from a conserved region of Pol.
 55. The vaccine of claim 54, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 751 or SEQ ID NO:
 753. 56. The vaccine of any one of claims 1-18, wherein the population episensus antigen is central to the HIV-1 2-clade regional epidemic in Thailand.
 57. The vaccine of claim 56, wherein the population episensus antigen comprises Gag epitopes.
 58. The vaccine of claim 56, wherein the population episensus antigen comprises epitopes from a conserved region of Gag.
 59. The vaccine of claim 58, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 60. The vaccine of claim 56, wherein the population episensus antigen comprises Nef epitopes.
 61. The vaccine of claim 56, wherein the population episensus antigen comprises epitopes from a conserved region of Nef.
 62. The vaccine of claim 56, wherein the population episensus antigen is a fusion antigen comprising two or more population episensus antigens.
 63. The vaccine of claim 62, wherein the fusion antigen comprises a population episensus antigen comprising Gag epitopes and a population episensus antigen comprising Nef epitopes.
 64. The vaccine of claim 62, wherein the fusion antigen comprises a population episensus antigen comprising epitopes from a conserved region of Gag and a population episensus antigen comprising epitopes from a conserved region of Nef.
 65. The vaccine of claim 64, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 66. The vaccine of claim 56, wherein the population episensus antigen comprises Pol epitopes.
 67. The vaccine of claim 56, wherein the population episensus antigen comprises epitopes from a conserved region of Pol.
 68. The vaccine of any one of claims 1-18, wherein the population episensus antigen is central to the HIV-1 M-group global set.
 69. The vaccine of claim 68, wherein the population episensus antigen comprises Gag epitopes.
 70. The vaccine of claim 69, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 718 or SEQ ID NO:
 720. 71. The vaccine of claim 68, wherein the population episensus antigen comprises epitopes from a conserved region of Gag.
 72. The vaccine of claim 71, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 73. The vaccine of claim 72, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 719 or SEQ ID NO:
 721. 74. The vaccine of claim 68, wherein the population episensus antigen comprises Nef epitopes.
 75. The vaccine of claim 74, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 722 or SEQ ID NO:
 724. 76. The vaccine of claim 68, wherein the population episensus antigen comprises epitopes from a conserved region of Nef.
 77. The vaccine of claim 76, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 723 or SEQ ID NO:
 725. 78. The vaccine of claim 68, wherein the population episensus antigen is a fusion antigen comprising two or more population episensus antigens.
 79. The vaccine of claim 78, wherein the fusion antigen comprises a population episensus antigen comprising Gag epitopes and a population episensus antigen comprising Nef epitopes.
 80. The vaccine of claim 79, wherein the fusion antigen comprises the amino acid sequence of SEQ ID NO: 705 or SEQ ID NO:
 707. 81. The vaccine of claim 78, wherein the fusion antigen comprises a population episensus antigen comprising epitopes from a conserved region of Gag and a population episensus antigen comprising epitopes from a conserved region of Nef.
 82. The vaccine of claim 81, wherein the epitopes from a conserved region of Gag are epitopes from the p24 capsid protein of Gag.
 83. The vaccine of claim 82, wherein the fusion antigen comprises the amino acid sequence of SEQ ID NO: 706 or SEQ ID NO:
 708. 84. The vaccine of claim 68, wherein the population episensus antigen comprises Pol epitopes.
 85. The vaccine of claim 84, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 709, SEQ ID NO: 711, SEQ ID NO: 726, or SEQ ID NO:
 728. 86. The vaccine of claim 68, wherein the population episensus antigen comprises epitopes from a conserved region of Pol.
 87. The vaccine of claim 86, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 710, SEQ ID NO: 712, SEQ ID NO: 727, or SEQ ID NO:
 729. 88. The vaccine of any one of claims 1-87, wherein the vaccine is a prophylactic vaccine.
 89. The vaccine of any one of claims 1-87, wherein the vaccine is a therapeutic vaccine.
 90. A polypeptide comprising one or more HIV-1 population episensus antigens determined using the EpiGraph approach, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 697-712 and SEQ ID NOs:717-753.
 91. A method of treating HIV-1 in a subject comprising administering an effective amount of the vaccine of any one of claims 1-87 to the subject in need thereof.
 92. A method of protecting from an HIV-1 infection in a subject comprising administering an effective amount of the vaccine of any one of claims 1-87 to the subject in need thereof.
 93. An HIV-1 vaccine comprising one or more carriers, a population episensus antigen determined using the EpiGraph approach, and one or more tailored antigens.
 94. The vaccine of claim 93, wherein the carrier is DNA, protein, or a vector
 95. The vaccine of claim 94, wherein the vector is a bacterial vector or a viral vector.
 96. The vaccine of claim 95, wherein the viral vector comprises a poxvirus, adenoviruses, rubella, sendai virus, rhabdovirus, alphavirus or adeno-associated virus backbone.
 97. The vaccine of claim 95, wherein the viral vector comprises a CMV backbone.
 98. The vaccine of claim 97, wherein the CMV backbone is a HCMV backbone or a RhCMV backbone.
 99. The vaccine of claim 97 or claim 98, wherein the CMV backbone lacks the UL130-128 gene region.
 100. The vaccine of any one of claims 97-99, wherein the CMV backbone lacks the UL82 gene encoding the tegument protein pp71.
 101. The vaccine of any one of claims 93-100, further comprising a second tailored antigen.
 102. The vaccine of claim 101, further comprising a third tailored antigen.
 103. The vaccine of any one of claims 97-102, wherein the population episensus antigen comprises Gag epitopes.
 104. The vaccine of claim 103, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO:
 691. 105. The vaccine of claim 103, wherein the population episensus antigen comprises the amino acid sequence of an inactivated Gag protein, wherein the amino acid sequence of the inactivated Gag protein was inactivated by eliminating a myristoylation sequence in the N-terminus of the Gag protein.
 106. The vaccine of any one of claims 93-105, wherein the tailored antigen comprises Gag epitopes.
 107. The vaccine of claim 106, wherein the tailored antigen comprises the amino acid sequence of SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695 or SEQ ID NO:
 696. 108. The vaccine of claim 107, wherein the first tailored antigen and the second tailored antigen comprise two of the amino acid sequences of SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695 and SEQ ID NO:
 696. 109. The vaccine of claim 108, wherein the first tailored antigen, the second tailored antigen, and the third tailored antigen comprise three of the amino acid sequences of SEQ ID NO: 692, SEQ ID NO: 693, SEQ ID NO: 694, SEQ ID NO: 695 and SEQ ID NO:
 696. 110. The vaccine of claim 106, wherein the tailored antigen comprises the amino acid sequence of an inactivated Gag protein, wherein the amino acid sequence of the inactivated Gag protein was inactivated by eliminating a myristoylation sequence in the N-terminus of the Gag protein.
 111. The vaccine of claim 93-102, wherein the population episensus antigen is central to the HIV-1 M-group global set.
 112. The vaccine of claim 111, wherein the population episensus antigen and the tailored antigen comprise Gag epitopes.
 113. The vaccine of claim 111, wherein the population episensus antigen and the tailored antigen sequence comprise epitopes form a conserved region of Gag.
 114. The vaccine of claim 113, wherein the epitopes from a conserved region of Gag are epitopes form the p24 capsid protein of Gag.
 115. The vaccine of any one of claims 112-114, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 754 or SEQ ID NO:
 755. 116. The vaccine of claim 115, wherein the tailored antigen comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 756-765.
 117. The vaccine of claim 116, wherein the first tailored antigen and the second tailored antigen comprise two of the amino acid sequences selected from the group consisting of: SEQ ID NOs: 756-765.
 118. The vaccine of claim 117, wherein the first tailored antigen, the second tailored antigen, and the third tailored antigen comprise three of the amino acid sequences selected from the group consisting of: SEQ ID NOs: 756-765.
 119. The vaccine of claim 93-102, wherein the population episensus antigen is central to the HIV-1 C clade epidemic in South Africa.
 120. The vaccine of claim 119, wherein the population episensus antigen and the tailored antigen comprise Gag epitopes.
 121. The vaccine of claim 119, wherein the population episensus antigen and the tailored antigen sequence comprise epitopes form a conserved region of Gag.
 122. The vaccine of claim 121, wherein the epitopes from a conserved region of Gag are epitopes form the p24 capsid protein of Gag.
 123. The vaccine of any one of claims 119-122, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 766 or SEQ ID NO:
 767. 124. The vaccine of claim 123, wherein the tailored antigen comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 768-777.
 125. The vaccine of claim 124, wherein the first tailored antigen and the second tailored antigen comprise two of the amino acid sequences selected from the group consisting of: SEQ ID NOs: 768-777.
 126. The vaccine of claim 125, wherein the first tailored antigen, the second tailored antigen, and the third tailored antigen comprise three of the amino acid sequences selected from the group consisting of: SEQ ID NOs: 768-777.
 127. The vaccine of claim 93-102, wherein the population episensus antigen is central to the HIV-1 B clade epidemic in South Africa.
 128. The vaccine of claim 127, wherein the population episensus antigen and the tailored antigen comprise Gag epitopes.
 129. The vaccine of claim 127, wherein the population episensus antigen and the tailored antigen sequence comprise epitopes form a conserved region of Gag.
 130. The vaccine of claim 129, wherein the epitopes from a conserved region of Gag are epitopes form the p24 capsid protein of Gag.
 131. The vaccine of any one of claims 127-130, wherein the population episensus antigen comprises the amino acid sequence of SEQ ID NO: 778 or SEQ ID NO:
 779. 132. The vaccine of claim 131, wherein the tailored antigen comprises an amino acid sequence selected from the group consisting of: SEQ ID NO: 780-789.
 133. The vaccine of claim 132, wherein the first tailored antigen and the second tailored antigen comprise two of the amino acid sequences selected from the group consisting of: SEQ ID NO: 780-789.
 134. The vaccine of claim 133, wherein the first tailored antigen, the second tailored antigen, and the third tailored antigen comprise three of the amino acid sequences selected from the group consisting of: SEQ ID NO: 780-789.
 135. The vaccine of any one of claims 93-102, wherein the population episensus antigen and the tailored antigen comprise Pol or Nef epitopes.
 136. The vaccine of any one of claims 93-135, further comprising a HCMV vector comprising a HCMV backbone and a tailored antigen selected to be a best match natural HIV-1 strain.
 137. The vaccine of any one of claims 93-136, further comprising a HCMV vector comprising a HCMV backbone and a tailored antigen selected to be a best match different (and distant) HIV-1 strain.
 138. The vaccine of any one of claims 93-137, wherein the vaccine is a therapeutic vaccine.
 139. An HIV-1 vaccine comprising one or more carriers and one or more population episensus antigens or tailored antigens, wherein the population episensus antigen and the tailored antigen are determined using the EpiGraph method.
 140. The vaccine of claim 139, wherein the carrier is DNA, protein, or a vector.
 141. The vaccine of claim 140, wherein the vector is a viral vector or a bacterial vector.
 142. The vaccine of claim 141, wherein the viral vector comprises a poxvirus, adenoviruses, rubella, sendai virus, rhabdovirus, alphavirus or adeno-associated virus backbone.
 143. The vaccine of claim 141, wherein the viral vector comprises a cytomegalovirus (CMV) backbone.
 144. The vaccine of claim 143, wherein the CMV backbone is a human CMV (HCMV) backbone or a rhesus CMV (RhCMV) backbone.
 145. The vaccine of claim 143 or 144, wherein the CMV backbone lacks the UL130-128 gene region.
 146. The vaccine of any one of claims 143-145, wherein the CMV backbone lacks the UL82 gene encoding the tegument protein pp71.
 147. The vaccine of any one of claims 139-146, wherein the population episensus antigen or the tailored antigen comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 691-696 and SEQ ID NOs: 754-789.
 148. The vaccine of claim 147, wherein the vaccine further comprises a second population episensus antigen or tailored antigen, and wherein the second population episensus antigen or tailored antigen comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 691-696 and SEQ ID NOs: 754-789.
 149. The vaccine of claim 148, wherein the vaccine further comprises a third population episensus antigen or tailored antigen, and wherein the third population episensus antigen or tailored antigen comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 691-696 and SEQ ID NOs: 754-789.
 150. The vaccine of any one of claims 139-149, wherein the vaccine is a therapeutic vaccine.
 151. A polypeptide comprising one or more HIV-1 population episensus antigens or HIV-1 tailored antigens, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 691-696 and SEQ ID NOs: 754-789.
 152. A method of treating HIV-1 in a subject comprising administering an effective amount of the vaccine of any one of claims 93-150 to the subject in need thereof.
 153. A method of designing and producing an HIV-1 vaccine for a subject comprising: (a) designing EpiGraph vaccine antigens to optimally cover the diversity within a geographic area using a vaccine antigen amino acid sequence generated using the EpiGraph method of antigen amino acid sequence selection; and, (b) producing said designed EpiGraph vaccine antigens.
 154. A method of designing and producing an HIV-1 vaccine for a subject comprising: (a) determining the amino acid sequence of HIV-1 viruses in an individual; (b) designing EpiGraph vaccine antigens to optimally cover the diversity within a geographic area using a vaccine antigen amino acid sequence generated using the EpiGraph method of antigen amino acid sequence selection; (c) producing said designed EpiGraph vaccine antigens; and (d) inserting the Epigraph vaccine antigens into a vector.
 155. A method of inducing an effector memory T cell response comprising: (a) designing one or more EpiGraph vaccine antigens; (b) producing a vaccine comprising the one or more EpiGraph vaccine antigens and a vector; and (c) administering the vaccine to a subject in need thereof.
 156. The method of any one of claims 153-155, wherein the EpiGraph vaccine antigen comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 691-712 and SEQ ID NOs: 717-789. 